High-resolution melting (HRM) analysis for the detection of single nucleotide polymorphisms in microRNA target sites

► We applied HRM analysis on the detection of SNPs in microRNA binding sites. ► We examined the SNPs in microRNA binding sites of PLA2G2A, IL-16 and NOD2. ► We examined the genotypes at different stages in acute lymphoblastic leukemia. ► HRM analysis is cost-effective in the detection of SNPs in microRNA binding sites.