Proteomic identification of serum biomarkers for gastric cancer using multi-dimensional liquid chromatography and 2D differential gel electrophoresis

► There is few research for finding serum biomarkers of gastric cancer by the technique of DIGE. ► We find combination of multi-dimensional HPLC and 2D-DIGE provides a valuable tool for serum proteomics in gastric cancer. ► We identify several proteins that were upregulated or decreased in gastric cancer. ► CLU may be a biomarker for diagnosis or prognosis of gastric cancer.