Preparation of highly purified monomeric human serum albumin as secondary reference material for standardization of urinary albumin immunoassays

► We prepared reference material for urine albumin immunoassay standardization. ► It consists of monomeric human serum albumin in lyophilized form ► The assigned concentration transferred from ERM-DA470 was 225.1 ± 9.11 mg/L ► A recalibration study demonstrated between-method CV of 6.6% using 129 urines ► The material is a powerful tool to standardize urine and total protein measurement.