Development of immunoassays for anti-electronegative LDL autoantibodies and immune complexes

► LDL(−) promotes atherosclerosis through inflammatory and immunologic mechanisms. ► We developed ELISAs to anti-LDL(−) autoantibodies and its immune complexes. ► The ELISAs showed precision and recovery within the required limits for immunoassays. ► These ELISAs can be used in clinical studies for investigation of atherosclerosis.