An automated method on analysis of blood steroids using liquid chromatography tandem mass spectrometry: Application to population screening for congenital adrenal hyperplasia in newborns

► We used the second tier steroid profile by LC-MS/MS in conjunction with EIA. ► The three steroid profile provided better screening outcomes of CAH than 17-OHP concentration alone. ► Our sample preparation allowed high throughput using common laboratory chemicals. ► Using three internal standards significantly improved method precision and accuracy. ► The reduction in false positives significantly reduces anxiety for newborns and their families.