Effects of a single dose of oral iron on hepcidin concentrations in human urine and serum analyzed by a robust LC-MS/MS method

► We report a robust quantification of hepcidin in urine and serum using UPLC-MS/MS. ► Serum iron, TfS, and serum and urinary hepcidin levels increase after FeSO4 [p < 0.05]. ► Oscillation of hepcidin levels in study subjects suggests a negative feed-back loop.