Evaluation of cytokeratin-1 in the diagnosis of hepatocellular carcinoma

► Cytokeratin-1 was identified at 67 kDa and quantified in sera of HCC patients using western blot and ELISA. ► MD analysis selected a score for the prediction of HCC vs liver cirrhosis patients based on levels of CK1, albumin and AFP. ► An area under ROC curves (AUC) of the score was 0.87. ► The score showed 87% sensitivity vs 39% sensitivity of AFP at cutoff value of 200 IU/ml for prediction of HCC from LC. ► Absolute specificity (100%) was obtained to discriminate HCC from healthy individual