کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8318836 1539118 2018 42 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid regulation of substrate use for oxidative phosphorylation during a single session of high intensity interval or aerobic exercises in different rat skeletal muscles
ترجمه فارسی عنوان
مقررات سریع استفاده سوبستات برای فسفوریلاسیون اکسیداتیو در یک جلسه فصلی با شدت بالا یا تمرین هوازی در عضلات اسکلتی مختلف موش صحرایی
کلمات کلیدی
ورزش، سازگاری عضلانی اسکلتی، میتوکندریا، اکسیداسیون پایه، ورزش با شدت بالا، تولید گونه های واکنشی اکسیژن و زیست زیستی میتوکندری،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Different exercise protocols lead to long-term adaptations that are related to increased mitochondrial content through the activation of mitochondrial biogenesis. However, immediate mitochondrial response to exercise and energetic substrate utilization is still unknown. We evaluate the mitochondrial physiology of two types rat skeletal muscle fibres immediately after a single session of high intensity interval exercise (HIIE) or aerobic exercise (AER). We found AER was able to reduce the ATP synthesis dependent oxygen flux in the tibialis (TA) when stimulated by complex I and II substrates. On the other hand, there was an increase of the maximum velocity (Vmax) for glycerol-phosphate oxidation and Vmax and affinity (KM) of palmitoyl-carnitine oxidation (PC). The exercise did not affect oxygen flux coupled to ATP synthesis in red gastrocnemius (RG) but, surprisingly, reduced its affinity for PC, decreasing the apparent catalytic efficiency (Vmax/KM) of oxidation for PC. Neither exercise protocol was able to change the electron transfer system capacity of the mitochondria or markers of mitochondrial content. The AER group had increased H2O2 production compared to the SED and HIIE groups, with the mechanism being predominantly the escape of electrons through reverse flux in complex I and other sites in TA, and only through other sites in RG. There were no changes in the activities of antioxidant enzymes. Our results show that mitochondria from different muscles submitted to distinct exercise protocols show alterations in the specific fluxes of substrate utilization and oxygen metabolism, indicating that the dynamics of mitochondria are linked to the metabolic flexibility.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 217, March 2018, Pages 40-50
نویسندگان
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