کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8319311 | 1539319 | 2015 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of thyroid hormone-dependent gene responses in vivo and in organ culture of the American bullfrog (Rana (Lithobates) catesbeiana) lung
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کلمات کلیدی
cold-inducible RNA binding proteinKLF9CIRBPTMSHSP30Thyroid hormone receptor alphathrb3,3′,5-triiodothyronineCATqPCRCu/Zn superoxide dismutase - Cu / Zn سوپر اکسید دیسموتازtricaine methane sulfonate - تری کینین متان سولفوناتOxidative stress - تنش اکسیداتیوLung - ریهSOD - سدMetamorphosis - متامورفیسمThyroid hormone - هورمون تیروئیدquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استCatalase - کاتالازThyroid hormone receptor beta - گیرنده هورمون تیروئید بتا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparison of thyroid hormone-dependent gene responses in vivo and in organ culture of the American bullfrog (Rana (Lithobates) catesbeiana) lung Comparison of thyroid hormone-dependent gene responses in vivo and in organ culture of the American bullfrog (Rana (Lithobates) catesbeiana) lung](/preview/png/8319311.png)
چکیده انگلیسی
Postembryonic frog development requires a thyroid hormone (TH)-dependent metamorphic transition from an aquatic larva to a terrestrial frog. Such change in environment involves lung maturation in preparation for breathing air. However, little is known regarding the underlying molecular events and the role of THs in this process. Using quantitative real-time polymerase chain reaction, we evaluated Rana (Lithobates) catesbeiana lung mRNA transcripts representing key elements of TH and oxidative stress signaling pathways during natural and TH-induced precocious metamorphosis. TH induction was evaluated in two ways: 1) in vivo through interperitoneal injection of 10 pmol/g body weight of 3,3â², 5-triiodothyronine (T3) into premetamorphic tadpoles and analysis after 48 h, and 2) in serum-free organ culture in the presence of 10 nM T3 after 48 h. Abundance of transcripts encoding the transcriptional regulators TH receptors α and β, TH-induced bZip protein, and CCAAT/enhancer binding protein 1 was increased during postembryonic development and following administration of exogenous THs to premetamorphic tadpoles in vivo and culture. In contrast, mRNA representing Krüppel-like factor 9 and cold-inducible RNA binding protein revealed differential effects between natural and precocious metamorphosis. Elevated levels of catalase and Cu/Zn superoxide dismutase mRNA were observed at the end of metamorphosis with transcript levels displaying minimal TH-dependency. No change in stress-responsive heat shock protein 30 mRNA abundance was noted. The results support a role for TH-dependent reprogramming of the lung transcriptome during frog development and reveal a requirement for increased antioxidant capacity following anuran metamorphosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 16, December 2015, Pages 99-105
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 16, December 2015, Pages 99-105
نویسندگان
Nik Veldhoen, Mitchel R. Stevenson, Caren C. Helbing,