کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8320833 1539408 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Insight into the cooperative DNA binding of the O6-alkylguanine DNA alkyltransferase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Insight into the cooperative DNA binding of the O6-alkylguanine DNA alkyltransferase
چکیده انگلیسی
The O6-alkylguanine DNA alkyltransferase (AGT) is a highly conserved protein responsible for direct repair of alkylated guanine and to a lesser degree thymine bases. While specific DNA lesion-bound complexes in crystal structures consist of monomeric AGT, several solution studies have suggested that cooperative DNA binding plays a role in the physiological activities of AGT. Cooperative AGT-DNA complexes have been described by theoretical models, which can be tested by atomic force microscopy (AFM). Direct access to structural features of AGT-DNA complexes at the single molecule level by AFM imaging revealed non-specifically bound, cooperative complexes with limited cluster length. Implications of cooperative binding in AGT-DNA interactions are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 20, August 2014, Pages 14-22
نویسندگان
, ,