کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322230 | 1539864 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of RNAs on chromatin accessibility and gene expression suggest RNA-mediated activation
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کلمات کلیدی
DMEMSDSeGFPALBFCSasRNARSBCentromereDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoAntisense RNA - RNA آنتیسنسAlbumin - آلبومینtris-EDTA buffer - بافر tris-EDTAchromatin accessibility - دسترسی کروماتینsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal calf serum - سرم گوساله جنینenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of RNAs on chromatin accessibility and gene expression suggest RNA-mediated activation Effects of RNAs on chromatin accessibility and gene expression suggest RNA-mediated activation](/preview/png/8322230.png)
چکیده انگلیسی
The study of the interaction between RNA and DNA sequences in activating genes has important significance for understanding the mechanisms of RNA-mediated activation. Here, we used in vitro chromatin reconstitution approach to observe whether RNAs increase DNase I digestion, plasmid transfection to observe whether RNAs promote gene expression, and bioinformatics analysis to predict the binding ability of RNAs to centromere DNA (constitutive heterochromatin). Synthetic RNAs (23Â nt) that were complementary to mouse albumin gene and total liver RNA increased DNase I digestion sensitivity of mouse albumin gene, suggesting that RNAs can increase chromatin accessibility. Transcribed sense-antisense tandem Alu elements activated an enhanced green fluorescent protein reporter gene after stable transfection. Bioinformatics analysis showed that the binding strength of RNA population to centromere DNAs is significantly lower than that of their flanking sequences, which suggests that the centromere is not easily affected by RNAs produced from other transcribed regions and may be the reason why centromeres consist of constitutive heterochromatin. The results in this paper illustrate that RNAs complementary to DNA sequences play roles in activating genes. Since RNA is mainly produced from the cell's own DNA, the work presented in this paper suggests that RNAs transcribed from DNA create feedback that activates DNA transcription.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 79, October 2016, Pages 24-32
Journal: The International Journal of Biochemistry & Cell Biology - Volume 79, October 2016, Pages 24-32
نویسندگان
Xiufang Wang, Zhihong Ma, Xianglong Kong, Zhanjun Lv,