کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322644 | 1539880 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of tandem E-box motifs within human Complement receptor 2 (CR2/CD21) promoter reveals cell specific roles for RP58, E2A, USF and localized chromatin accessibility
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کلمات کلیدی
CHART-PCRbHLHIEF2DE - 2deElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزbasic helix-loop-helix - اسلحه پایه حلقه ایtwo dimensional electrophoresis - الکتروفورز دو بعدیHuman - انسانchromatin immunoprecipitation - ایمن سازی کروماتینisoelectric focusing - تمرکز ذره ای الکتریکیGene regulation - تنظیم ژنB cells - سلول های BEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکTranscription factor - عامل رونویسیnuclear extract - عصاره هسته ایTranscription factors - عوامل رونویسیcage - قفسMALDI-TOF MS - مالدی توف MSMatrix-assisted laser desorption/ionization time of flight mass spectrometry - مدت زمان جذب / زمان یونیزاسیون طیف سنجی جرمی پرواز با کمک ماتریکسCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Analysis of tandem E-box motifs within human Complement receptor 2 (CR2/CD21) promoter reveals cell specific roles for RP58, E2A, USF and localized chromatin accessibility Analysis of tandem E-box motifs within human Complement receptor 2 (CR2/CD21) promoter reveals cell specific roles for RP58, E2A, USF and localized chromatin accessibility](/preview/png/8322644.png)
چکیده انگلیسی
Complement receptor 2 (CR2/CD21) plays an important role in the generation of normal B cell immune responses. As transcription appears to be the prime mechanism via which surface CR2/CD21 expression is controlled, understanding transcriptional regulation of this gene will have broader implications to B cell biology. Here we report opposing, cell-context specific control of CR2/CD21 promoter activity by tandem E-box elements, spaced 22Â bp apart and within 70Â bp of the transcription initiation site. We have identified E2A and USF transcription factors as binding to the distal and proximal E-box sites respectively in CR2-positive B-cells, at a site that is hypersensitive to restriction enzyme digestion compared to non-expressing K562 cells. However, additional unidentified proteins have also been found to bind these functionally important elements. By utilizing a proteomics approach we have identified a repressor protein, RP58, binding the distal E-box motif. Co-transfection experiments using RP58 overexpression constructs demonstrated a specific 10-fold repression of CR2/CD21 transcriptional activity mediated through the distal E-box repressor element. Taken together, our results indicate that repression of the CR2/CD21 promoter can occur through one of the E-box motifs via recruitment of RP58 and other factors to bring about a silenced chromatin context within CR2/CD21 non-expressing cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 64, July 2015, Pages 107-119
Journal: The International Journal of Biochemistry & Cell Biology - Volume 64, July 2015, Pages 107-119
نویسندگان
Mark N. Cruickshank, James Dods, Rhonda L. Taylor, Mahdad Karimi, Emily J. Fenwick, Elizabeth A. Quail, Alexander J. Rea, V. Michael Holers, Lawrence J. Abraham, Daniela Ulgiati,