کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322804 | 1539884 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of stage-specific markers during differentiation of hair cells from mouse inner ear stem cells or progenitor cells in vitro
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کلمات کلیدی
EGFOHCsIGF-1DAPIHCART-PCRSSCbFGFFSCPBS4′6-diamidino-2-phenylindole - 4'6-diamidino-2-phenylindoleinsulin-like growth factor-1 - انسولین مانند عامل رشد 1Hierarchical cluster analysis - تجزیه و تحلیل خوشه سلسله مراتبیMicroarray analysis - تجزیه و تحلیل میکرواراDifferentiation - تفکیکStem cells - سلول های بنیادیHair cells - سلول های مویOuter hair cells - سلول های موی بیرونیprogenitor cells - سلول های پیش گیاهepidermal growth factor - عامل رشد اپیدرمیbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریBMP - مدیریت فرایند کسب و کارGene ontology - هستیشناسی ژنیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسforward scatter - پراکندگی رو به جلوside scatter - پراکندگی سمتBone morphogenetic protein - پروتئین مورفوژنیک استخوان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The induction of inner ear hair cells from stem cells or progenitor cells in the inner ear proceeds through a committed inner ear sensory progenitor cell stage prior to hair cell differentiation. To increase the efficacy of inducing inner ear hair cell differentiation from the stem cells or progenitor cells, it is essential to identify comprehensive markers for the stem cells/progenitor cells from the inner ear, the committed inner ear sensory progenitor cells and the differentiating hair cells to optimize induction conditions. Here, we report that we efficiently isolated and expanded the stem cells or progenitor cells from postnatal mouse cochleae, and induced the generation of inner ear progenitor cells and subsequent differentiation of hair cells. We profiled the gene expression of the stem cells or progenitor cells, the inner ear progenitor cells, and hair cells using aRNA microarray analysis. The pathway and gene ontology (GO) analysis of differentially expressed genes was performed. Analysis of genes exclusively detected in one particular cellular population revealed 30, 38, and 31 genes specific for inner ear stem cells, inner ear progenitor cells, and hair cells, respectively. We further examined the expression of these genes in vivo and determined that Gdf10+Ccdc121, Tmprss9+Orm1, and Chrna9+Espnl are marker genes specific for inner ear stem cells, inner ear progenitor cells, and differentiating hair cells, respectively. The identification of these marker genes will likely help the effort to increase the efficacy of hair cell induction from the stem cells or progenitor cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 60, March 2015, Pages 99-111
Journal: The International Journal of Biochemistry & Cell Biology - Volume 60, March 2015, Pages 99-111
نویسندگان
Quanwen Liu, Jiarong Chen, Xiangli Gao, Jie Ding, Zihua Tang, Cui Zhang, Jianling Chen, Liang Li, Ping Chen, Jinfu Wang,