کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8324068 | 1539903 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lipid raft regulates the initial spreading of melanoma A375 cells by modulating β1 integrin clustering
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کلمات کلیدی
ECMDMEMFBSMmpsCTXBMβCD3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTsiRNA - siRNA4-morpholineethanesulfonic acid - اسید 4-مورفولینتان سولفونیکsmall interference RNA - تداخل کوچک RNAEMT - تکنسین فوریتهای پزشکیfetal bovine serum - سرم جنین گاوLipid raft - قایق های چربExtracellular matrix - ماتریکس خارج سلولیMatrix metalloproteinases - متالوپروتئیناز ماتریکسmethyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینMeS - مسAdaptor proteins - پروتئین آداپتورFocal adhesion - چسبندگی کانونیcholera toxin subunit B - کلسترول توکسین BEpithelial–mesenchymal transition - گذار اپیتلیال-مزانشیمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cell adhesion and spreading require integrins-mediated cell-extracellular matrix interaction. Integrins function through binding to extracellular matrix and subsequent clustering to initiate focal adhesion formation and actin cytoskeleton rearrangement. Lipid raft, a liquid ordered plasma membrane microdomain, has been reported to play major roles in membrane motility by regulating cell surface receptor function. Here, we identified that lipid raft integrity was required for β1 integrin-mediated initial spreading of melanoma A375 cells on fibronectin. We found that lipid raft disruption with methyl-β-cyclodextrin led to the inability of focal adhesion formation and actin cytoskeleton rearrangement by preventing β1 integrin clustering. Furthermore, we explored the possible mechanism by which lipid raft regulates β1 integrin clustering and demonstrated that intact lipid raft could recruit and modify some adaptor proteins, such as talin, α-actinin, vinculin, paxillin and FAK. Lipid raft could regulate the location of these proteins in lipid raft fractions and facilitate their binding to β1 integrin, which may be crucial for β1 integrin clustering. We also showed that lipid raft disruption impaired A375 cell migration in both transwell and wound healing models. Together, these findings provide a new insight for the relationship between lipid raft and the regulation of integrins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 8, August 2013, Pages 1679-1689
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 8, August 2013, Pages 1679-1689
نویسندگان
Ruifei Wang, Jiajia Bi, Khamal Kwesi Ampah, Chunmei Zhang, Ziyi Li, Yang Jiao, Xiaoru Wang, Xueqing Ba, Xianlu Zeng,