Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail

► Non-muscle myosin IIA is involved in Golgi remodeling. ► Non-muscle myosin IIA transports Golgi proteins to ER for proteasomal degradation. ► Inhibition of non-muscle myosin IIA prevents brefeldin A-induced Golgi collapse. ► Inhibition of heat shock proteins distinguishes Golgi matrix and non-matrix proteins.