کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326010 | 1539954 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased spermine oxidase (SMO) activity as a novel differentiation marker of myogenic C2C12 cells
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کلمات کلیدی
FCMTGMC2C12 muscle cellsSPDMCKODCOrnithine decarboxylaseTDMC2C12ASMASPMIGF-1SMOROS - ROSsSAT - SSATSpermine - اسپرمSpermidine - اسپرمیدینspermine oxidase - اسپرمین اکسیدازdifferentiation medium - تمایز رسانهCell differentiation - تمایز سلولیFlow cytometric - جریان سیاتومتریmuscle creatine kinase - عضله کراتین کینازInsulin-like growth factor - فاکتور رشد مانند انسولینputrescine - قرار دادنPUT - قرار دادنGrowth medium - محیط رشدPropidium iodide - پروتئین یدیدPolyamines - پلی آمینAPAO - پیوستReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Increased spermine oxidase (SMO) activity as a novel differentiation marker of myogenic C2C12 cells Increased spermine oxidase (SMO) activity as a novel differentiation marker of myogenic C2C12 cells](/preview/png/8326010.png)
چکیده انگلیسی
Spermine oxidase (SMO) is a FAD-containing enzyme involved in animal cell polyamines (PA) homeostasis, selectively active on spermine and producing H2O2, spermidine, and the 3-aminopropanal. In the present study, we have examined the SMO gene expression during the mouse myoblast C2C12 cell differentiation induced with two different stimuli by RT-PCR analysis, polysome-mRNP distribution and enzyme activity. SMO transcript accumulation and enzymatic activity increases during C2C12 cell differentiation and correlates with the decrease of spermine content. Many proteins are highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated post-mitotic myotubes. The SMO gene induction represents a novel and additional marker of C2C12 cell differentiation. The sub-cellular localization of the SMOα and SMOμ splice variants is not involved in the differentiation processes. Nuclear localization of only the SMOμ protein was confirmed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 41, Issue 4, April 2009, Pages 934-944
Journal: The International Journal of Biochemistry & Cell Biology - Volume 41, Issue 4, April 2009, Pages 934-944
نویسندگان
Manuela Cervelli, Emiliano Fratini, Roberto Amendola, Marzia Bianchi, Emanuela Signori, Elisabetta Ferraro, Antonella Lisi, Rodolfo Federico, Lucia Marcocci, Paolo Mariottini,