کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326588 | 1539977 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the West Nile virus protease substrate specificity and inhibitors
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کلمات کلیدی
BAPNAPBSWNVDENV-2AmCDFPAprotinin7-amino-4-methyl coumarin - 7 آمینو 4-متیل کومارینDMSO - DMSOamino acid - آمینو اسیدFluorescence assay - تست فلورسانسSubstrate specificity - خصوصیات زیربناییDimethyl sulfoxide - دیمتیل سولفواکسیدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMolecular modeling - مدل سازی مولکولیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازDengue virus type 2 - ویروس دوگانه نوع 2West Nile virus - ویروس نیل غربی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
West Nile virus (WNV), a mosquito-borne member of Flaviviridae, is a human pathogen causing widespread disease for which there is no vaccine or chemotherapy. The two-component viral serine protease consists of a heterodimeric complex between the hydrophilic domain of the cofactor, NS2B (NS2BH) and the protease domain (NS3-pro). The protease is essential for polyprotein processing followed by assembly of viral replicase and genome replication. Therefore, the protease is an excellent target for development of antiviral therapeutics. Here, we report the expression in Escherichia coli, purification, and characterization of biochemical and kinetic properties of the WNV protease. Furthermore, we show that the WNV and the dengue virus type 2 (DENV-2) proteases are inhibited by aprotinin with inhibitor constants of 0.16 and 0.026 μM, respectively. Molecular modeling of the WNV protease/aprotinin complex, based on the known crystal structures of the WNV NS2BH-N3pro and aprotinin, suggest a potentially strong interaction between the P2 Lys and the protease activator peptide, NS2BH. This conclusion based on molecular modeling is in agreement with our data of a higher kcat/Km value with the substrate, Boc-Gly-Lys-Arg-MCA than the Boc-Gly-Arg-Arg-MCA and is also consistent with the results of an earlier study that were based on substrate-based inhibitor peptides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 39, Issue 3, 2007, Pages 606-614
Journal: The International Journal of Biochemistry & Cell Biology - Volume 39, Issue 3, 2007, Pages 606-614
نویسندگان
Niklaus H. Mueller, Changsuek Yon, Vannakambadi K. Ganesh, R. Padmanabhan,