کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326623 | 1540195 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced thermostability of halo-tolerant glutaminase from Bacillus licheniformis ATCC 14580 by immobilization onto nano magnetic cellulose sheet and its application in production of glutamic acid
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
A halo-tolerant glutaminase gene (BlglsA) was isolated from Bacillus licheniformis. Heterologous expression of BlglsA revealed that it encodes for a 36â¯kDa protein containing 327 amino acid residues. The purified enzyme showed optimal activity at a pH of 9.5 while 35â¯Â°C was found to be the optimum temperature. The enzyme retained about 92 and 97% stability at pHâ¯12 and temperature (40â¯Â°C) respectively. Subsequent immobilization of BlglsA on nano magnetic cellulose sheet (NMCS) led to an enhanced tolerance to higher temperature. NMCS-BlglsA showed optimum activity at 45â¯Â°C, although it was stable even at 60â¯Â°C. NaCl tolerance (â¥90% in 0.3â¯M) was almost similar to BlglsA and NMCS-BlglsA. The metal ions Fe2+ (5â¯mM) and Mn2+ (2.5â¯mM) improved the BlglsA relative activity by 61 and 48%, respectively. In contrast, 5â¯mM Mn2+ was found suitable to enhance the activity of NMCS-BlglsA up to 72%. The production of glutamic acid by NMCS-BlglsA was 1.61â¯g/l in 48â¯h. Reusability test of NMCS-BlglsA showed 76 and 35% retention of the actual activity after 4th and 7th cycle, respectively. Such remarkable biochemical properties of NMCS-BlglsA make it an attractive enzyme for food industries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 119, November 2018, Pages 1256-1263
Journal: International Journal of Biological Macromolecules - Volume 119, November 2018, Pages 1256-1263
نویسندگان
Ram Baskaran, Ramesh Bandikari, Wu Zuo, Jiaxin Qian, Ziduo Liu,