کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327654 | 1540201 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of antioxidant and antiglycated activities of polysaccharides from Arthrocnemum indicum leaves
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves. Three independent variables were opted: extraction temperature, time and ratio (solvent/material). The optimal settings were: extraction temperature of 80 °C, time of 288 min and (solvent/solid) ratio of 40 mL/g. Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81 mg ascorbic acid equivalents/g matter and 69.81%, respectively. Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively. Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis. SEC analyzes revealed that polysaccharide had an average molecular weight of 2179 kDa. Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively. This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 113, 1 July 2018, Pages 774-782
Journal: International Journal of Biological Macromolecules - Volume 113, 1 July 2018, Pages 774-782
نویسندگان
Zeineb Mzoughi, Mohamed Aymen Chaouch, Khaoula Mkadmini Hammi, Jawhar Hafsa, Didier Le Cerf, Riadh Ksouri, Hatem Majdoub,