کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8328180 | 1540205 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression, purification, and characterization of a novel amylosucrase from Neisseria subflava
ترجمه فارسی عنوان
بیان، تصفیه و مشخص کردن یک آمیلوسوکراس رمان از سوپرایوئوس نایسریا
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Amylosucrase (ASase) is a glucosyltransferase, which catalyzes the de novo synthesis of amylose-like polymers from sucrose. In the present study, ASase from Neisseria subflava (NsAS) was cloned, sequenced, and expressed in Escherichia coli. The production of NsAS was achieved by inducting gene expression with 0.2â¯mM isopropyl-β-d-thiogalactopyranoside. The molecular mass of the Ni-NTA column purified NsAS analyzed by SDS-PAGE was determined to be 72â¯kDa. NsAS exhibited maximal activity at 45â¯Â°C and pH 8.0, and showed strong thermal stability at 40â¯Â°C with a half-life of 385â¯h. The reaction pattern of NsAS at [sucrose] range of 0.1-1.0â¯M showed that at 0.7â¯M of [sucrose], the production yield of insoluble linear α-(1,4)-glucans reached 24% maximum, and any further increase in [sucrose] resulted in a slight decrease in yield. Meanwhile, the production yield of turanose significantly increased from 16 to 29% by increasing [sucrose] from 0.1 to 1.0â¯M. The synthesized glucan had degrees of polymerization (DP); for 0.1, 0.4, 0.7, and 1.0â¯M sucrose, the DP values were 77, 49, 39, and 31 respectively. These results suggested that NsAS would be a promising candidate for food industrial production of linear α-(1,4)-glucans and turanose as a next generation sweetener.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 109, 1 April 2018, Pages 160-166
Journal: International Journal of Biological Macromolecules - Volume 109, 1 April 2018, Pages 160-166
نویسندگان
Min-Oh Park, Murugesan Chandrasekaran, Sang-Ho Yoo,