کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8328323 | 1540205 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of laccase on epoxy-functionalized silica and its application in biodegradation of phenolic compounds
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Immobilization of laccase on epoxy-functionalized silica and its application in biodegradation of phenolic compounds Immobilization of laccase on epoxy-functionalized silica and its application in biodegradation of phenolic compounds](/preview/png/8328323.png)
چکیده انگلیسی
A novel method of laccase immobilization on epoxy-functionalized silica particles was developed. Laccase from Myceliophthora thermophila was covalently immobilized onto epoxy-functionalized matrix by nucleophilic attack of amino groups of laccase to epoxy groups of the support. The enzyme loading on the support was about 30â¯mg/g under the optimum conditions (pH 4.5, 24â¯h). The effect of pH, temperature and organic solvent on immobilized enzyme activity was determined and compared with those of free enzyme. In general the immobilized enzyme was found to be stabilized compared to the free enzyme. Lineweaver-Burk plots were used to calculate kinetic parameters for ABTS oxidation. KM values were 24.0 and 25.3â¯Î¼M while vmax values were 10.0 and 1.6â¯Î¼Mâ¯minâ1 for free and immobilized laccase, respectively. The performance of the biocatalyst was evaluated by the degradation of phenolic compounds including phenol, p-chlorophenol and catechol. The removal efficiency of catechol by immobilized laccase was about 95% after 2â¯h.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 109, 1 April 2018, Pages 443-447
Journal: International Journal of Biological Macromolecules - Volume 109, 1 April 2018, Pages 443-447
نویسندگان
Mehdi Mohammadi, Mohammad Ali As'habi, Peyman Salehi, Maryam Yousefi, Mahboobeh Nazari, Jesper Brask,