کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8328626 | 1540207 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic purification and structure characterization of glucuronoxylan from water extract of Cassia obtusifolia seeds
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کلمات کلیدی
SECHPAECCassia obtusifoliaGC–MS - GC-MSNMR - تشدید مغناطیسی هستهای Nuclear magnetic resonance (NMR) - رزونانس مغناطیسی هسته ای (NMR)gas chromatography–mass spectroscopy - طیف سنجی جرم کروماتوگرافی گازHigh-performance anion-exchange chromatography - عملکرد بالا کروماتوگرافی مبدل آنیونیEnzymatic hydrolysis - هیدرولیز آنزیمیweight-average molecular weight - وزن مولکولی متوسطSize exclusion chromatography - کروماتوگرافی اندازهای طردیGlucuronoxylan - گلوکورونوکسیلان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enzymatic hydrolysis was proposed for the first time to purify the sub-fraction of water-extracted cassia polysaccharide (CP-40), and a homogeneous polysaccharide CP-40-M was obtained with relatively high yield. The structural features of CP-40-M were characterized using size-exclusion chromatography equipped with multiple detectors (SEC-MALLS), high-performance anion-exchange chromatography (HPAEC), methylation and gas chromatography-mass (GC-MS), as well as nuclear magnetic resonance (NMR) spectra. The weight-average molecular weight for CP-40-M was determined to be 0.29 Ã 105 Da, and the molar ratio of xylose to glucuronic acid was 4.62. The structure of CP-40-M was elucidated to be glucuronoxylan, with glucopyranosyluronic acid group terminally attached to O-2 of the â4)-β-Xylp-(1â backbone. It was the first time to obtain this type of xylan from the water extract of Cassia obtusifolia seeds. The structure elucidation of CP-40-M was meaningful for better understanding the natural characteristics of cassia polysaccharide and important for their potential use in food industry and folk medicine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 107, Part B, February 2018, Pages 1438-1446
Journal: International Journal of Biological Macromolecules - Volume 107, Part B, February 2018, Pages 1438-1446
نویسندگان
Lei Feng, Jun-Yi Yin, Shao-Ping Nie, Yi-Qun Wan, Ming-Yong Xie,