Extracting xylooligosaccharides in wheat bran by screening and cellulase assisted enzymatic hydrolysis

Functional xylooligosaccharides (XOS) from wheat bran were carried out by screening and cellulase assisted enzymatic hydrolysis. First, wheat bran was crushed and screened, getting the optimal experimental particle size of raw materials (>125 μm), and 72.43% of starch. Wheat bran was then repeated colloid milled and hydrolysed with cellulase, releasing about 45% of xylan (higher than the untreated wheat bran (about 29%)). Finally, membrane treatment (20 kDa and 1 kDa cutoff) was chosen to eliminate impurities and enriched XOS. In the designed process, 51 mg purified XOS was obtained from 1 g dry wheat bran powder.