کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8329964 | 1540238 | 2016 | 54 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimized extraction and molecular characterization of polysaccharides from Sophora alopecuroides L. seeds
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Optimized extraction of crude polysaccharides from Sophora alopecuroides L. seeds (CSAP) was firstly investigated, and the results were extraction time 4.15 h, extraction temperature 70.68 °C and water/seed ratio 50.99:1 ml/g. At this proximal point, highest yield (10.88%) and relative viscosity (1.2952) were obtained for CSAP, which contained 78.40% sugar, 6.08% protein, 9.71% ash, 7.16% moisture and 1.88% uronic acid. Neutral fractions of F15, F25, F35 and F60 were further obtained via stepwise alcohol precipitation. Results of monosaccharide composition indicated that CSAP and its neutral fractions mainly consisted of mannose and galactose (79.45-97.52%) with M/G ratios of 0.94-1.48, typical for galactomannans, which were demonstrated by FT-IR. Weight-average molecular weight, radius of gyration and hydrodynamic radius were in the same order (F15 > F25 > F35 > F60), whereas the highest intrinsic viscosity was observed for F25. Multiple analyses, including Mark-Houwink-Sakurada exponent (0.48-0.80), persistence length (1.45-3.93 nm), conformation zoning (random coil) and power-law exponent (b > 1), showed flexible coils for all neutral fractions. Concerning viscometry, there were no aggregates (Kâ³ 0.60) or coil overlaps (“master curve”) of F25 in dilute solutions. Relatively weak surface activity of F25 was probably related to its particular chemical structure and proteinaceous moieties closely conjoined.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 231-242
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 231-242
نویسندگان
Rui Guo, Nannan Cao, Yan Wu, Jinhong Wu,