کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8330261 1540239 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides
چکیده انگلیسی
Polysaccharide extraction from Dipsacus asperoides roots (DAP) was proved to possess strong antioxidant activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities, inhibiting β-carotene bleaching and strong reducing power. Cell assay demonstrated that the crude DAP possessed antioxidant activity and were effective against H2O2-induced L02 cells injury. Then, response surface methodology (RSM) was applied to optimize the ultrasonic extraction of DAP. The optimum variables given by central composite design (CCD) were as follows: ratio of water to raw material, 38.61 mL/g; ultrasonic power, 308.68 W; extraction time, 38.61 min; and extraction temperature, 89 °C. Under these conditions, the maximum yield of DAP obtained was 7.12 ± 0.45%. Moreover, high performance liquid chromatography (HPLC) analysis suggested that the monosaccharide compositions of DAP contained primarily mannose, ribose, glucose, galactose, xylose and arabinose, with a molar ratio of 0.22:0.48:2.29:0.34:1.39:1.41. The results of the present study showed that DAP could be considered as potential sources of natural antioxidants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 81, November 2015, Pages 332-339
نویسندگان
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