کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8330921 | 1540241 | 2015 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of an Aspergillus niger xylanase in yeast: Application in breadmaking and in vitro digestion
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The cDNA of the β-1,4-endoxylanase of Aspergillus niger US368 was cloned and expressed in Pichia pastoris under the constitutive GAP promoter. The maximum activity obtained was 41 U mLâ1, which was about 3-fold higher than that obtained with the native species. The purified enzyme showed a specific activity of 910 U mgâ1 and a molecular mass of 24 kDa. It had an optimal activity at pH 4 and 50 °C, stable in a wide range of pH and in the presence of some detergents and organic solvents. r-XAn11-His6 (recombinant xylanase) was used as an additive in breadmaking. A decrease in water absorption, an increase in dough rising and improvements in volume and specific volume of the bread were recorded. The r-XAn11-His6 was also used in in vitro digestion of barley and wheat bran leading to a decrease of the viscosities and an increase of the reducing sugars and total sugars contents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 103-109
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 103-109
نویسندگان
Fatma Elgharbi, Aïda Hmida-Sayari, Youssef Zaafouri, Samir Bejar,