کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8330925 | 1540241 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of the I682F mutation on JAK2's activity, structure and stability
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کلمات کلیدی
Janus Kinase 2EmaxB-ALLMPDS1-anilino-8-naphthalenesulfonic acidJanus kinase - کیناز جانوس myeloproliferative disorders - اختلالات میلوپرولیفراتیوStructural stability - ثبات ساختاریANS - سالB-cell acute lymphoblastic leukemia - لوسمی لنفوبلاستی حاد B-سلولیAcute lymphoblastic leukemia - لوسمی لنفوبلاستیک حادwild type - نوع وحشیALL - همهJAK - چگونه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of the I682F mutation on JAK2's activity, structure and stability Effects of the I682F mutation on JAK2's activity, structure and stability](/preview/png/8330925.png)
چکیده انگلیسی
Janus kinase 2 (JAK2) plays important roles in the regulation of varieties cellular processes including cell migration, proliferation and apoptosis. JAK2 I682F genetic mutation existed in the 4-8% of B-cell acute lymphoblastic leukemia (B-ALL). However, roles of this mutation in the development of B-ALL are still unknown. In order to investigation the mechanism of the JAK2 I682F mutation led to B-ALL, series of mutations were constructed. Mutations I682F, I682G, I682D and I682S significantly increased JAK2's activity and decreased its structural stability, while the I682L mutation almost had no effect on JAK2's activity and structural stability. Furthermore, the spectroscopic experiments implied that mutations I682F, I682G, I682D and I682S impaired the structure of JAK2 JH2 domain, and led JAK2 to the partially unfolded state. It may be this partially unfolded state that caused JAK2 I682F constitutive activation. This study provides clues in understanding the mechanism of the JAK2 I682F mutation caused B-ALL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 118-125
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 118-125
نویسندگان
Feng Li, Wei Li, Yang Liu, Yu-Xue Tong, Ping Zhou, Lin Wang, Chong Chen, Ling-Yu Zeng, Qing-Yun Wu, Xiao-Yun Wang, Kai-Lin Xu,