کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8331014 1540241 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel glucose oxidase biosensor based on poly([2,2′;5′,2″]-terthiophene-3′-carbaldehyde) modified electrode
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A novel glucose oxidase biosensor based on poly([2,2′;5′,2″]-terthiophene-3′-carbaldehyde) modified electrode
چکیده انگلیسی
In the study, the electrochemical behavior of glucose oxidase (GOx) immobilized on poly([2,2′;5′,2″]-terthiophene-3′-carbaldehyde) (poly(TTP)) modified glassy carbon electrode (GCE) was investigated. The biosensor (poly(TTP)/GOx/GCE) showed a pair of redox peaks in 0.1 M phosphate buffer (pH 7.4) solution in the absence of oxygen the co-substrate of GOx. In here, Poly(TTP)/GOx/GCE biosensor acts as the co-substrate instead of oxygen. Upon the addition of glucose, the reduction and oxidation peak currents increased until the active site of GOx was fully saturated with glucose. The apparent m was estimated 26.13 mM from Lineweaver-Burk graph. The biosensor displayed a good stability and bioactivity. The biosensor showed a high sensitivity (56.1 nA/mM), a linear range (from 0.5 to 20.15 mM), and a good reproducibility with 3.6% of relative standard deviation. In addition, the interference currents of glycin, ascorbic acid, histidine, uric acid, dopamine, arginine, and fructose on GOx biosensor were investigated. All that substances exhibited an interference current under 10%. It was not shown a marked difference between GOx biosensor and spectrophotometric measurement of glucose in serum examples. UV-visible spectroscopy and scanning electron microscopy (SEM) experiments of the biosensor were also performed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 262-268
نویسندگان
, , ,