کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8331196 | 1540242 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
l-rhamnose-binding lectin from eggs of the Echinometra lucunter: Amino acid sequence and molecular modeling
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: l-rhamnose-binding lectin from eggs of the Echinometra lucunter: Amino acid sequence and molecular modeling l-rhamnose-binding lectin from eggs of the Echinometra lucunter: Amino acid sequence and molecular modeling](/preview/png/8331196.png)
چکیده انگلیسی
An l-rhamnose-binding lectin named ELEL was isolated from eggs of the rock boring sea urchin Echinometra lucunter by affinity chromatography on lactosyl-agarose. ELEL is a homodimer linked by a disulfide bond with subunits of 11 kDa each. The new lectin was inhibited by saccharides possessing the same configuration of hydroxyl groups at C-2 and C-4, such as l-rhamnose, melibiose, galactose and lactose. The amino acid sequence of ELEL was determined by tandem mass spectrometry. The ELEL subunit has 103 amino acids, including nine cysteine residues involved in four conserved intrachain disulfide bonds and one interchain disulfide bond. The full sequence of ELEL presents conserved motifs commonly found in rhamnose-binding lectins, including YGR, DPC and KYL. A three-dimensional model of ELEL was created, and molecular docking revealed favorable binding energies for interactions between ELEL and rhamnose, melibiose and Gb3 (Galα1-4Galβ1-4Glcβ1-Cer). Furthermore, ELEL was able to agglutinate Gram-positive bacterial cells, suggesting its ability to recognize pathogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 78, July 2015, Pages 180-188
Journal: International Journal of Biological Macromolecules - Volume 78, July 2015, Pages 180-188
نویسندگان
Rômulo Farias Carneiro, Claudener Souza Teixeira, Arthur Alves de Melo, Alexandra Sampaio de Almeida, Benildo Sousa Cavada, Oscarina Viana de Sousa, Bruno Anderson Matias da Rocha, Celso Shiniti Nagano, Alexandre Holanda Sampaio,