کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8331841 | 1540245 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine Enhanced thermal stability and pH behavior of glucose oxidase on electrostatic interaction with polyethylenimine](/preview/png/8331841.png)
چکیده انگلیسی
Electrostatic interactions, mediated by ionic-exchange, between polyethylenimine (PEI) and glucose oxidase (GOx) were used to form GOx-PEI macro-complex, which were evaluated for pH and thermal stability of GOx. Under the experimental conditions, the complex had a dominant GOx presence on its surface and a hydrodynamic diameter of 205 ± 16 nm. Activity was evaluated from 40 to 75 °C, and at pH from 2 to 12. GOx activity in complex was maintained up to 70 °C and it was lost at 75 °C. In contrast, free GOx showed a maximum activity at 50 °C, which was completely lost at 70 °C. This difference, observed by fluorescence analysis, was associated with the compact unfolded structure of GOx in the complex. This GOx stability was not observed under pH variations, and complex formation was only possible at pH â¥Â 5 where enzymatic activity was diminished by the presence of PEI.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 75, April 2015, Pages 453-459
Journal: International Journal of Biological Macromolecules - Volume 75, April 2015, Pages 453-459
نویسندگان
Silvia G. Padilla-MartÃnez, LucÃa MartÃnez-Jothar, José G. Sampedro, Ferdinando Tristan, ElÃas Pérez,