کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333851 | 1540264 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase
ترجمه فارسی عنوان
شتاب ژله سازی و ارتقاء کانی سازی هیدروژل های کیتوزان توسط آلکالن فسفاتاز
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کلمات کلیدی
کیتوزان، معدنی شدن، کامپوزیت، هیدروژل،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Thermosensitive chitosan hydrogels containing sodium beta-glycerophosphate (β-GP), whose gelation is induced by increasing temperature to body temperature, were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone. ALP incorporation led to acceleration of gelation upon increase of temperature for four different chitosan preparations of differing molecular weight, as demonstrated by rheometric time sweeps at 37 °C. Hydrogels containing ALP were subsequently incubated in calcium glycerophosphate (Ca-GP) solution to induce their mineralization with calcium phosphate (CaP) in order to improve their suitability as materials for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP mineral, as confirmed by SEM, FTIR, Raman spectroscopy, XRD, ICP-OES, and increases in dry mass percentage, which rose with increasing ALP concentration and incubation time in Ca-GP solution. The results demonstrate that ALP accelerates formation of thermosensitive chitosan/β-GP hydrogels and induces their mineralization with CaP, which paves the way for applications as injectable bone replacement materials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 56, May 2013, Pages 122-132
Journal: International Journal of Biological Macromolecules - Volume 56, May 2013, Pages 122-132
نویسندگان
Timothy E.L. Douglas, Agata Skwarczynska, Zofia Modrzejewska, Lieve Balcaen, David Schaubroeck, Sylvia Lycke, Frank Vanhaecke, Peter Vandenabeele, Peter Dubruel, John A. Jansen, Sander C.G. Leeuwenburgh,