Solution and in silico studies on the recombinant lectin from Cicer arietinum seeds

► Cloning and expression of the plant lectin gene into active protein in Escherichia coli. ► Thermo-labile protein showing rapid structural changes from 50 to 55 °C. ► Unfolding followed by dissociation of the dimer in presence of GdnHCl. ► Reorientation of trp microenvironment in low concentration of GdnHCl. ► Experimental results correlate well with the homology model.