دانلود مقالات ISI درباره Gdnhcl + ترجمه فارسی

قطره مایع پروتئین پلی الکترولیت برای تشکیل ترکیبات با غلظت بالا

Liquid Droplet of Protein-Polyelectrolyte Complex for High-Concentration Formulations

Keywords: complexation; dissolution; polyelectrolyte(s); precipitation; protein formulation(s); phase separation(s); protein(s); CD; circular dichroism; GdnHCl; guanidine hydrochloride; pI; isoelectric point; polyE; poly-l-glutamic acid; polyE1; polyE of 1.5-5.5

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2018 سفارش ترجمه

تأثیر و تأثیر اسمولیت ها در فرمولاسیون بیولوژیک

Influence and effect of osmolytes in biopharmaceutical formulations

Keywords: Biologics; Protein formulation; Stabilisers; Excipients; Vaccine; BSA; bovine serum albumin; BCG; Bacillus Calmette - Guérin; Cyt c; cytochrome c; F; folded; GdnHCl; guanidine hydrochloride; IgG; immunoglobulin G; IgM; immunoglobulin M; FDA; food and d

دانلود رایگان متن کامل مقاله ISI 31 صفحه سال انتشار : 2018 سفارش ترجمه

مشخصات فیزیکوشیمیایی آلبومین سرم انسان از اسیدهای کولی و مقایسه آن با پلاکت همسان انسانی نشان می دهد که آن را به عنوان بیوسیمیل امیدوار کننده

Physicochemical characterization of E. coli-derived human serum albumin and its comparison with the human plasma counterpart reveals it as a promising biosimilar

Keywords: FA; fatty acid; GdnHCl; guanidium hydrochloride; HSA; human serum albumin; pHSA; plasma-derived human serum albumin; rHSA; recombinant human serum albumin; Recombinant human serum albumin; Escherichia coli; Recombinant biosimilar;

دانلود رایگان متن کامل مقاله ISI 35 صفحه سال انتشار : 2018 سفارش ترجمه

اثرات تراکم ماکرومولکولی بر روی آشکار شدن، ترکیب و ثبات قلیایی فسفاتاز

Effects of macromolecular crowding on alkaline phosphatase unfolding, conformation and stability

Keywords: ANS; 8-anilino-1-naphthalenesulfonic acid; ALP; alkaline phosphatase; GdnHCl; guanidine hydrochloride; Tris; tris(hydroxymethyl) aminomethane; CD; circular dichroism; p-NPP; p-nitrophenyl phosphate; UV-vis; ultraviolet-visible spectroscopy; Macromolec

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2017 سفارش ترجمه

بیو سازگاری مایعات یونی با ثبات پروتئین: یک مرور جامع در مورد درک فعلی و پیامدهای آن

Biocompatibility of ionic liquids towards protein stability: A comprehensive overview on the current understanding and their implications

Keywords: ILs; Ionic Liquids; IL; Ionic Liquid; AAs; Amino acids; AA; Amino acid; CT; α-chymotrypsin; Mb; Myoglobin; BSA; Bovine serum albumin; HSA; Human serum albumin; PGA; Penicillin G acylase; ADH; Alcohol dehydrogenase; CALB; Candida antarctica lipase B; rPA;

دانلود رایگان متن کامل مقاله ISI 134 صفحه سال انتشار : 2017 سفارش ترجمه

اختلالات سازگاری پروتئین در آمیلوئیدوز ارثی: اثر دیفرانسیل جهش تک نقطه در انواع آمیلویدوژن آپوای

Protein conformational perturbations in hereditary amyloidosis: Differential impact of single point mutations in ApoAI amyloidogenic variants

Keywords: ANS; 8-anilino-1-naphthalensulfonic acid; ApoAI; apolipoprotein A I; AApoAI; amyloidogenic apolipoprotein A I; CD; circular dichroism; DLS; dynamic light scattering; GdnHCl; guanidine hydrochloride; HDL; high-density lipoproteins; MD; molecular dynamics;

دانلود رایگان متن کامل مقاله ISI 37 صفحه سال انتشار : 2016 سفارش ترجمه

دنباله روان در حال ظهور لکتین موز: حالت های مونومرهای گسسته، انعکاسی و انعطاف پذیر مانند گوانیدین هیدروکلراید

Novel unfolding sequence of banana lectin: Folded, unfolded and natively unfolded-like monomeric states in guanidine hydrochloride

Keywords: Banana lectin; Unfolding sequence; Structured intermediate; Random coil; Natively unfolded state; Banlec; banana lectin; GdnHCl; guanidine hydrochloride; ANS; 8-anilino-1-naphthalenesulfonate; NBS; N-bromosuccinimide; PBS; 10 mM sodium phosphate buffered

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2014 سفارش ترجمه

بازسازی پروتئین نوترکیب اسم29، یک گام در جهت تولید کاندیدا واکسن علیه اسکیزوستیکوزیس است

Refolding of the recombinant protein Sm29, a step toward the production of the vaccine candidate against schistosomiasis

Keywords: HHP; high hydrostatic pressure; GSSG and GSH; oxidized and reduced form of glutathione* respectively; IB; inclusion bodies; CD; circular dichroism; IMAC; immobilized metal affinity chromatography; LS; light scattering; GdnHCl; guanidine hydrochloride; Pro

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

بینش های مربوط به مکانیزم مولکولی تجمع پروتئین بومی مانند گلایکاسیون

Insights into the molecular mechanism of protein native-like aggregation upon glycation

Keywords: α-CHCA; α-Cyano-4-hydroxicinamic acid; ACN; Acetonitrile; AGE; Advanced glycation end-product; AIC; Akaike Information Criteria; CID; Collision induced dissociation; GdnHCl; Guanidinium hydrochloride; MAGE; Methylglyoxal-derived advanced glycation end-p

دانلود رایگان متن کامل مقاله ISI 47 صفحه سال انتشار : 2013 سفارش ترجمه

Introduction of an extra tryptophan fluorophore by cataract-associating mutations destabilizes βB2-crystallin and promotes aggregation

Keywords: βB2-crystallin; Congenital hereditary cataract; Protein aggregation; Protein folding; Trp fluorophore; UV damage; ANS; 1-anilinonaphtalene-8-sulfonate; CD; circular dichroism; DTT; dithiothreitol; Emax; maximum emission wavelength of fluorescence; IPTG;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2018 سفارش ترجمه

Ordered opening of LDL receptor binding domain of human apolipoprotein E3 revealed by hydrogen/deuterium exchange mass spectrometry and fluorescence spectroscopy

Keywords: Apolipoprotein E3; Helix bundle opening; LDL receptor; Hydrogen deuterium exchange mass spectrometry; Fluorescence spectroscopy; Lipoprotein binding; apoE3; apolipoprotein E3; CD; circular dichroism; CT; C-terminal; GdnHCl; guanidine hydrochloride; FI; fl

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2018 سفارش ترجمه

Minichaperone (GroEL191-345) mediated folding of MalZ proceeds by binding and release of native and functional intermediates

Keywords: SPR; surface plasmon resonance; TBR; transient binding and release; ANS; 8-anilino-1-naphthalene sulfonate; GdnHCl; guanidine hydrochloride; Tm; temperature of melting; MalZ; Maltodextrin gluocosidase; Protein folding; Minichaperone assisted protein foldi

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2018 سفارش ترجمه

Leptospira interrogans thermolysin refolded at high pressure and alkaline pH displays proteolytic activity against complement C3

Keywords: GdnHCl; guanidine hydrochloride; HHP; high hydrostatic pressure; IPTG; isopropyl β-D-thiogalactopyranoside; LB; Luria-Bertani broth; LS; light scattering; TL-IB; inclusion bodies of thermolysin; SDS-PAGE; sodium dodecyl sulphate polyacrylamide gel electr

دانلود رایگان متن کامل مقاله ISI 20 صفحه سال انتشار : 2018 سفارش ترجمه

Evaluation of in vitro refolding vs cold shock expression: Production of a low yielding single chain variable fragment

Keywords: Inclusion bodies; Single chain variable fragment; In vitro protein refolding; Cold shock; Disulfide bridge; Tissue factor; Fusion partner; Codon Adaptation Index; CAI; Dimethyl sulfoxide; DMSO; E.coli Codon Usage Analyzer; ECUA; Escherichia coli; E.coli;

دانلود رایگان متن کامل مقاله ISI 27 صفحه سال انتشار : 2018 سفارش ترجمه

The cataract-causing mutation G75V promotes γS-crystallin aggregation by modifying and destabilizing the native structure

Keywords: ADCC; autosomal dominant congenital cataract; ANS; 1-anilinonaphtalene-8-sulfonate; BSA; bovine serum albumin; CD; circular dichroism; DTT; dithiothreitol; Emax; maximum emission wavelength of intrinsic Trp fluorescence; GdnHCl; guanidine hydrochloride; I

دانلود رایگان متن کامل مقاله ISI 31 صفحه سال انتشار : 2018 سفارش ترجمه

Highly efficient production of functional recombinant human fibroblast growth factor 22 in E. coli and its protective effects on H2O2-lesioned L02 cells

Keywords: rhFGF22; Inclusion body; Two-step denaturing method; Protective effects; Mitochondrial apoptotic pathways; FGF; human fibroblast growth factor; rhFGF22; recombinant human FGF22; FGFR; FGF receptors; PTZ; pentylenetetrazol; ROS; reactive oxygen species; Gd

دانلود رایگان متن کامل مقاله ISI 33 صفحه سال انتشار : 2018 سفارش ترجمه

Folding and stabilizing membrane proteins in amphipol A8-35

Keywords: 3D; three-dimensional; A8-35; a polyacrylate-based amphipol; APol; amphipol; BO; bacterio-opsin; BR; bacteriorhodopsin from Halobacterium salinarum; Ca2+-ATPase; the sarcoplasmic calcium pump; cac; critical aggregation concentration; CD; circular dichrois

دانلود رایگان متن کامل مقاله ISI 42 صفحه سال انتشار : 2018 سفارش ترجمه

Structural plasticity of T4 transcription co-activator gp33 revealed by a protease-resistant unfolded state

Keywords: gp33; Protease resistance; Protein-urea interaction; Structural plasticity; <λ>; average wavelength; BSA; bovine serum albumin; CD; circular dichroism; Cm; mid-point of chemical denaturation; fU; unfolded fraction; GdnHCl; guanidine hydrochloride; gp33;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2017 سفارش ترجمه

Characterization of the second conserved domain in the heme uptake protein HtaA from Corynebacterium diphtheriae

Keywords: ABC transporter; ATP-binding cassette transporter; CD; circular dichroism; CO; carbon monoxide; CR; conserved region; CT; charge-transfer; CV; column volume; DMSO; dimethyl sulfoxide; ELISA; enzyme-linked immunosorbent assay; ESI; electrospray ionization;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2017 سفارش ترجمه

pH induced single step shift of hydrophobic patches followed by formation of an MG state and an amyloidogenic intermediate in Lima Bean Trypsin Inhibitor (LBTI)

Keywords: LBTI; Lima Bean Trypsin Inhibitor; MG; molten globule; PPI; protein protease inhibitor; PI; protease inhibitor; Ia; amyloidiogenic intermediate; Y69; tyrosine 69; E70; glutamate 70; GdnHCl; guanidine hydrochloride; TEM; transmission electron microscopy; C

دانلود رایگان متن کامل مقاله ISI 35 صفحه سال انتشار : 2017 سفارش ترجمه

Functional and conformational transitions of mevalonate diphosphate decarboxylase from Bacopa monniera

Keywords: Mevalonate diphosphate decarboxylase; Unfolding; Aggregation; Fluorescence quenching; CD spectroscopy; BmMDD; Bacopa monniera mevalonate diphosphate decarboxylase; CD; circular dichroism; GdnHCl; Guanidine hydrochloride; MEP; 2-C methyl-d-erythritol-4-pho

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2016 سفارش ترجمه

Epoxide based inhibitors of the hepatitis C virus non-structural 2 autoprotease

Keywords: HCV; hepatitis C virus; NS; non-structural; SGR; subgenomic replicon; TPCK; tosyl phenylalanine chloromethyl ketone; GdnHCl; guanidine hydrochloride; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate; DTT; dithiothreitol; JFH1; Japanese Fu

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2015 سفارش ترجمه

Non-two-state thermal denaturation of ferricytochrome c at neutral and slightly acidic pH values

Keywords: cyt c; ferricytochrome c; GdnHCl; guanidine hydrochloride; CD; circular dichroism; DSC; differential scanning calorimetry; Protein stability; Conformational change; Ligand exchange; Alkaline state; Thermal denaturation;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2015 سفارش ترجمه

Native state dynamics affects the folding transition of porcine pancreatic phospholipase A2

Keywords: N; native state; U; unfolded state; D; denaturant; GdnHCl; guanidinium chloride; GdnHSCN; guanidinium isothiocyanate; ΔSASA; change of solvent accessible surface area; PLA2; phospholipase A2; ppPLA2; porcine pancreatic phospholipase A2; bpPLA2; bovine pa

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2015 سفارش ترجمه

Probing selected structural regions in the secreted phospholipase A2 from Arabidopsis thaliana for their impact on stability and activity

Keywords: Secreted phospholipase A2 from Arabidopsis thaliana; Trp variants; Structure-stability relationship; Activity; N-Terminal deletion; AtPLA2α; sPLA2α from Arabidopsis thaliana; bpPLA2; sPLA2 from bovine pancreas; bvPLA2; sPLA2 from bee venom; DOPC; 1,2-

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Structural dynamic and thermodynamic analysis of calcineurin B subunit induced by calcium/magnesium binding

Keywords: CN; calcineurin; CNB; calcineurin B subunit; CNA; calcineurin A; NFAT; nuclear factor of activated T cells; FKBP12; the FK506-binding protein; CaBPs; calcium binding proteins; IPTG; isopropyl thiogalactopyranoside; CD; circular dichroism; GdnHCl; guanidin

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Solution and in silico studies on the recombinant lectin from Cicer arietinum seeds

Keywords: Cicer lectin; BL21-CodonPlus (DE3)-RIL cells; Steady-state and time resolved fluorescence; Unfolding; Aggregation; Homology model; rCAL; recombinant Cicer arietinum lectin; GdnHCl; guanidine hydrochloride; ANS; 8-anilino-1-napthalenesulphonate;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

The amino acid residue L113 is involved in arginine kinase activity and structural stability

Keywords: AK; arginine kinase; CK; creatine kinase; PK; phosphogen kinase; IPTG; isopropyl-d-thiogalactopyranoside; ANS; 1-anilinonaphtalene-8-sulfonate; SEC; size exclusion chromatography; GdnHCl; guanidine hydrochloride; Emax; emission maximum wavelength of the i

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Protein l-isoaspartyl-O-methyltransferase of Vibrio cholerae: Interaction with cofactors and effect of osmolytes on unfolding

Keywords: Methyltransferase; Folding intermediate; Osmolytes; Protein modeling and simulation; AdoMet; S-adenosyl methionine; AdoHcy; S-adenosyl homocysteine; bis-ANS; 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid; CD; circular dichroism; DLS; dynamic l

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

The N-terminal fragment of Acanthamoeba polyphaga mimivirus tyrosyl-tRNA synthetase (TyrRSapm) is a monomer in solution

Keywords: TyrRSapm; Acanthamoeba polyphaga mimivirus tyrosyl-tRNA synthetase; ΔTyrRSapm; N-terminal domain of Acanthamoeba polyphaga mimivirus tyrosyl-tRNA synthetase; aa; amino acid; AUC; analytical ultracentrifuge; DTNB; 5,5′-dithio-bis(2-nitrobenzoic acid); D

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Exploring the unfolding mechanism of γ-glutamyltranspeptidases: The case of the thermophilic enzyme from Geobacillus thermodenitrificans

Keywords: ANS; 1-anilino-8-naphthalenesulfonate; BlGT; γ-glutamyltranspeptidase from Bacillus licheniformis; BsGT; γ-glutamyltranspeptidase from Bacillus subtilis; CD; circular dichroism; GdnHCl; guanidinium hydrochloride; EcGT; γ-glutamyltranspeptidase from Esc

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Differing structural characteristics of molten globule intermediate of peanut lectin in urea and guanidine-HCl

Keywords: PNA; peanut agglutinin; ANS; 8-anilino-1-naphthalenesulfonate; NBS; N-bromosuccinimide; GdnHCl; guanidine hydrochloride; PBS; 20 mM sodium phosphate buffered with 0.15 M NaCl, pH 7.2; CD; circular dichroism; HPLC; high performance liquid chromatography;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Identification of an active dimeric intermediate populated during the unfolding process of the cambialistic superoxide dismutase from Streptococcus mutans

Keywords: Superoxide dismutase; Thermal stability; Chemical stability; Dimeric intermediate; Unfolding; ANS; 1-anilino-8-naphthalene sulfonate; BsSOD; superoxide dismutase (SOD) from Bacillus subtilis; C1/2; concentration of denaturant at midpoint denaturation; EcS

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Impact of subunit and oligomeric structure on the thermal and conformational stability of chlorite dismutases

Keywords: Cld; chlorite dismutase; NdCld; Candidatus “Nitrospira defluvii”; apo-NdCld; heme-free chlorite dismutase from Candidatus “Nitrospira defluvii”; NwCld; chlorite dismutase from Nitrobacter winogradskyi; DSC; differential scanning calorimetry; ECD;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2012 سفارش ترجمه

Effects of osmolytes on human brain-type creatine kinase folding in dilute solutions and crowding systems

Keywords: CK; creatine kinase; HBCK; human brain-type creatine kinase; rHBCK; recombinant HBCK; GdnHCl; guanidine hydrochloride; PEG 2000; polyethylene glycol 2000; CT DNA; calf thymus DNA; λmax; maximum emission wavelength; Human brain-type creatine kinase; Macro

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2012 سفارش ترجمه

Comparative analysis of malate synthase G from Mycobacterium tuberculosis and E. coli: Role of ionic interaction in modulation of structural and functional properties

Keywords: MtbMS; Mycobacterium tuberculosis malate synthase G; ecMS; E. Coli malate synthase G; SEC; size exclusion chromatography; GdnHCl; guanidine hydrochloride; CD; circular dichroism; GRASP; graphical representation and analysis of structural properties; Domai

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2011 سفارش ترجمه

Influence of N-terminal helix bundle stability on the lipid-binding properties of human apolipoprotein A-I

Keywords: ABCA1; ATP-binding cassette transporter A1; ANS; 8-anilino-1-naphthalenesulfonic acid; apo; apolipoprotein; CD; circular dichroism; DMPC; dimyristoylphosphatidylcholine; GdnHCl; guanidine hydrochloride; HDL; high-density lipoprotein; HX; hydrogen-deuter

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2011 سفارش ترجمه

Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point

Keywords: ANS; 1-anilino-8-naphthalene sulfonic acid; CD; circular dichroism; GdnHCl; guanidine hydrochloride; MG; molten globule; NATA; N-acetyl-l-tryptophanamide; SBM; stem bromelain; SMG; specific molten globule; TFE; 2,2,2-trifluoroethanol; ThT; thioflavin T; A

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2011 سفارش ترجمه

Folding kinetic pathway of phosphofructokinase-2 from Escherichia coli: A homodimeric enzyme with a complex domain organization

Keywords: Phosphofructokinase; Folding pathway; Subunit association; Bimolecular domain; Ribokinase-like family; Subunit interface; Folding kinetics; Pfk-2; phosphofructokinase-2; GdnHCl; guanidine hydrochloride; DTT; dithiotreitol; CD; circular dichroism scpectros

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2011 سفارش ترجمه

Pea lectin unfolding reveals a unique molten globule fragment chain

Keywords: Pea lectin; Equilibrium unfolding; Fluorescence; Phosphorescence; Tryptophan modification; PSL; Pea (Pisum sativum) lectin; ANS; 8-anilino-1-naphthalenesulfonate; NBS; N-bromosuccinimide; GdnHCl; guanidine hydrochloride; REES; red edge excitation shift; H

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2011 سفارش ترجمه

Structure and stability of crustacean lipovitellin: Influence of lipid content and composition

Keywords: Lipoprotein; Crustacean; Phospholipase A2; Fluorescence quenching; Lipids; GdnHCl;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2010 سفارش ترجمه

Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate

Keywords: Abz-AGLA-Nba; 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide; GdnHCl; guanidine hydrochloride; kobs; observed rate constant; pWT; pseudo-wild type; TCA; trichloroacetic acid; Tris; tris(hydroxymethyl)aminomethane; ΔG#; Gibbs free energy of activation;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2010 سفارش ترجمه

Conformational stability and energetics of Plasmodium falciparum glutaredoxin

Keywords: Denaturation; Equilibrium; Redox protein; Stability; Unfolding; Grx(s); Glutaredoxin(s); PfGrx; Plasmodium falciparum Glutaredoxin; PfGLP1; Plasmodium falciparum glutaredoxin like protein-1; ESI-MS; Electrospray ionization mass spectrometry; GSH; Reduced

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2010 سفارش ترجمه

Reduced stability and enhanced surface hydrophobicity drive the binding of apo-aconitase with GroEL during chaperone assisted refolding

Keywords: ANS; 1-anilino naphthalene-8-sulfonate; GdnHCl; guanidine hydrochloride; MG; molten globule; N; native; U; unfolded; PK; proteinase K; Apo-aconitase; Holo-aconitase; Aconitase unfolding; GroEL-assisted folding; Metallo protein biosynthesis;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2010 سفارش ترجمه

A comparative analysis of the fluorescence properties of the wild-type and active site mutants of the hepatitis C virus autoprotease NS2-3

Keywords: HCV; Hepatitis C virus; NS; non-structural; JFH-1; Japanese fulminant hepatitis C virus genotype 2a isolate; WT; wild-type; GdnHCl; guanidine hydrochloride; DTT; dithiothreitol; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; CD; circula

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2010 سفارش ترجمه

Stability enhancement of cytochrome c through heme deprotonation and mutations

Keywords: GdnHCl; guanidine hydrochloride; PA c551; cytochrome c551 from Pseudomonas aeruginosa; CD; circular dichroism; Circular dichroism spectroscopy; Cytochrome c; Hydrogen bond; Protein stability; Heme propionate;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2009 سفارش ترجمه

Oligomerisation and thermal stability of polyvalent integrin α5β1 ligands

Keywords: DTT; dithiothreitol; GdnHCl; guanidine hydrochloride; s20,b; experimental sedimentation coefficient for the protein(s) at 293 K in buffer; s20,w0; experimental sedimentation coefficient corrected to standard conditions and extrapolated to infinite diluti

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2009 سفارش ترجمه

Mutation of the conserved Asp122 in the linker impedes creatine kinase reactivation and refolding

Keywords: ANS; 1-anilinonaphtalene-8-sulfonate; CK; creatine kinase; RMCK; rabbit muscle CK; CD; circular dichroism; Cr; creatine; CTD; carboxyl-terminal domain of CK; GdnHCl; guanidine hydrochloride; NTD; amino-terminal domain of CK; PCr; phosphocreatine; SEC; siz

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Reversible unfolding of dimeric phosphofructokinase-2 from Escherichia coli reveals a dominant role of inter-subunit contacts for stability

Keywords: Pfk-2; phosphofructokinase-2; GdnHCl; guanidine hydrochloride; ANS; 8-anilino-1-naphthalene sulfonic acid; DTT; dithiotreitol; SEC; size exclusion chromatography; CD; circular dichroism spectroscopy; DLS; dynamic light scattering; Cm; guanidine hydrochlor

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

The effect of additional disulfide bonds on the stability and folding of ribonuclease A

Keywords: AUAA; 6-carboxyfluorescein-dArU(dA)2-6-carboxytetramethylrhodamine; DTNB; 5,5′-dithiobis-(2-nitrobenzoic acid); GdnHCl; guanidine hydrochloride; ONC; onconase; RNase; ribonuclease; Ribonuclease A (EC 3.1.27.5); Onconase (EC 3.1.27.5); Ribonuclease A; Th

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2009 سفارش ترجمه