Functional and conformational changes in the aspartic protease cardosin A induced by TFE

► We studied the effects of TFE on cardosin A activity and structure. ► Low TFE concentrations induce a decrease on stability and a reversible activity loss. ► Medium TFE concentrations inactivated cardosin A and lead to an increase on structure complexity. ► MD simulation show significant local alterations on cardosin A flexibility.