کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8335136 | 1540277 | 2012 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Halophilic characterization of starch-binding domain from Kocuria varians α-amylase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Halophilic characterization of starch-binding domain from Kocuria varians α-amylase Halophilic characterization of starch-binding domain from Kocuria varians α-amylase](/preview/png/8335136.png)
چکیده انگلیسی
The tandem starch-binding domains (KvSBD) located at carboxy-terminal region of halophilic α-amylase from moderate halophile, Kocuria varians, were expressed in E. coli with amino-terminal hexa-His-tag and purified to homogeneity. The recombinant KvSBD showed binding activity to raw starch granules at low to high salt concentrations. The binding activity of KvSBD to starch was fully reversible after heat-treatment at 85 °C. Circular dichroism and thermal scanning experiments indicated that KvSBD showed fully reversible refolding upon cooling after complete melting at 70 °C in the presence of 0.2-2.0 M NaCl. The refolding rate was enhanced with higher salt concentration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 1, 1 January 2012, Pages 95-102
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 1, 1 January 2012, Pages 95-102
نویسندگان
Rui Yamaguchi, Yasuhiro Inoue, Hiroko Tokunaga, Matsujiro Ishibashi, Tsutomu Arakawa, Jun-ichi Sumitani, Takashi Kawaguchi, Masao Tokunaga,