کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8335518 | 1540279 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of lysozyme with gold nanorods: conformation and activity investigations
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Interaction of lysozyme with gold nanorods: conformation and activity investigations Interaction of lysozyme with gold nanorods: conformation and activity investigations](/preview/png/8335518.png)
چکیده انگلیسی
Gold nanorods, with their unique morphology and optical properties have offered new prospects for biomedical and biosensing applications. Herein, the interaction between gold nanorods and a model protein has been monitored using spectroscopic techniques. The enzyme retains high fraction of its native structure with a slight increase in the helical content at the expense of β-turns. Kinetic investigations revealed notable increase of enzyme affinity for substrate without significant decrease in the Vmax. Emission spectra of tryptophan residues in the presence of chaotropic agent highlighted the maintenance of internal quenching due to the induced compactness. Comparison of the gold nanorod treated lysozyme with free enzyme revealed higher thermodynamic stability under denaturing condition. Results from this study encourage the possibility of utilizing gold nanorods as promising nanocarrier candidates for a new generation of drug delivery applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 49, Issue 4, 1 November 2011, Pages 629-636
Journal: International Journal of Biological Macromolecules - Volume 49, Issue 4, 1 November 2011, Pages 629-636
نویسندگان
Tahereh Tohidi Moghadam, Bijan Ranjbar, Khosro Khajeh, Seyed Masoud Etezad, Khosrow Khalifeh, Mohamad Reza Ganjalikhany,