کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8335704 | 1540279 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cobalt(III)-induced hexamerization of PEGylated insulin
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کلمات کلیدی
IBMXSE-HPLCtmaxEC50RP-HPLCmPEGPEGylationDPBSMALDI-TOFFBSDMEM3-isobutyl-1-methylxanthine - 3-ایزوبوتیل-1-متیلکسانتینDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoSize-exclusion high-performance liquid chromatography - اندازه گیری کروماتوگرافی مایع با کارایی بالاfetal bovine serum - سرم جنین گاوDulbecco's phosphate-buffered saline - فسفات باسیل نمک Dulbeccomatrix-assisted laser desorption/ionization time of flight - مدت زمان جذب / یونیزاسیون لیزر ماتریس پروازCobalt - کبالتReversed phase high performance liquid chromatography - کروماتوگرافی مایع با عملکرد بالا فاز برگشت پذیر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Insulin conjugates in which the B1Phe residue has been chemically modified often exhibit a reduced tendency to associate into hexamers due to weakened interactions between subunits. The purpose of this study was to prepare a hexamer formulation for such insulin conjugates by using Co(III) as a coordinating metal ion. PEGylated insulin in which monomethoxypoly(ethylene glycol) (mPEG, Mr 5000 or 20,000) had been site-specifically attached to B1Phe was chosen as a model conjugate. Hexamerization of mPEG-insulin upon H2O2-mediated oxidation of Co(II) was kinetically and quantitatively analysed by visible spectrometry and size-exclusion HPLC. Co(III) mPEG-insulin hexamers thus obtained were extremely stable, existing mostly as a hexameric form even at nanomolar concentrations. A remarkable increase in hydrodynamic volumes was observed for Co(III) mPEG(20k)-insulin hexamers (1600Â kDa), as well as Co(III) mPEG(5k)-insulin hexamers (300Â kDa). Our results demonstrate the potential benefits of Co(III) hexamer formulation for weakly associating insulin conjugates in the treatment of diabetes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 49, Issue 4, 1 November 2011, Pages 832-837
Journal: International Journal of Biological Macromolecules - Volume 49, Issue 4, 1 November 2011, Pages 832-837
نویسندگان
Sung In Lim, Myung Hyun Jang, Dae Jin Kim, Sung Min Bae, Se Chang Kwon,