کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336770 | 1540643 | 2015 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor γ with miR-27 involved mechanism
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The effects of four proanthocyanidin dimers including epicatechin-(4βâ8, 2βâOâ7)-epicatechin (A-type EC dimer), epicatechin-(4βâ8)-epicatechin (B-type EC dimer), epicatechin-3-gallate-(4βâ8, 2βâOâ7)-epicatechin-3-gallate (A-type ECG dimer) and epigallocatechin-3-gallate-(4βâ8, 2βâOâ7)-epigallocatechin-3-gallate (A-type EGCG dimer) on 3T3-L1 preadipocyte cell differentiation and the underlying mechanisms were explored and compared. The results showed that A-type ECG dimer and A-type EGCG dimer significantly reduced the intracellular lipid accumulation in 3T3-L1 preadipocyte cells by targeting miR-27a and miR-27b as well as peroxisome proliferator-activated receptor γ (PPARγ) in the early stage of differentiation, while A-type EC dimer and B-type EC dimer showed little effect. In addition, our results revealed that the inhibitory effects of proanthocyanidin dimers on 3T3-L1 preadipocyte differentiation were highly structure-dependent and the effects were associated with the dimer-membrane interactions. The presence of galloyl moieties and A-type linkage within the structure of proanthocyanidins might be crucial for their inhibitory effect on adipogenesis. The strong disturbing effects of A-type ECG and A type EGCG dimers on the fluidity, hydrophobicity and permeability of membrane of 3T3-L1 preadipocyte cell were at least, in part, responsible for their distinct inhibitory effects on adipocyte hyperplasia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 26, Issue 11, November 2015, Pages 1124-1135
Journal: The Journal of Nutritional Biochemistry - Volume 26, Issue 11, November 2015, Pages 1124-1135
نویسندگان
Wei Zhu, Bo Zou, Rongzu Nie, Ying Zhang, Chun-mei Li,