کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8337656 | 1540686 | 2012 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purple corn anthocyanins dampened high-glucose-induced mesangial fibrosis and inflammation: possible renoprotective role in diabetic nephropathy
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کلمات کلیدی
ECMMT1-MMPTGFPBS-TMCP-1CTGFRT-PCRICAM-1HRMCFBSDMEMMMPMTT - MTTPCA - PCAROS - ROStissue inhibitors of matrix metalloproteinases - بازدارنده های بافت متالوپروتئیناز ماتریکسtransforming growth factor - تبدیل فاکتور رشدDulbecco's modified Eagle's media - رسانه های عقاب اصلاح شده DulbeccoTIMP - زمانfetal bovine serum - سرم جنین گاوConnective tissue growth factor - فاکتور رشد بافت همبندrenal fibrosis - فیبروز کلیویExtracellular matrix - ماتریکس خارج سلولیMatrix metalloproteinases - متالوپروتئیناز ماتریکسmembrane type-1 matrix metalloproteinase - متالوپروتئیناز ماتریکس نوع 1 غشاییDiabetic nephropathy - نفروپاتی دیابتیReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایmonocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1high glucose - گلوکز بالا یا قند بالاReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purple corn anthocyanins dampened high-glucose-induced mesangial fibrosis and inflammation: possible renoprotective role in diabetic nephropathy Purple corn anthocyanins dampened high-glucose-induced mesangial fibrosis and inflammation: possible renoprotective role in diabetic nephropathy](/preview/png/8337656.png)
چکیده انگلیسی
Purple corn has been classified as a functional food rich in anthocyanins possessing potential disease-preventive properties. This study examined whether purple corn anthocyanins (PCA) mainly comprised cyanidin 3-glucoside and cyanidin-3-(6â³-malonylglucoside) can attenuate high-glucose (HG)-promoted mesangial cell (MC) proliferation and matrix accumulation, major features of diabetic glomerulosclerosis. Human renal MC were cultured for 3 days in media containing 5.5 mM glucose plus 27.5 mM mannitol as osmotic controls or media containing 33 mM glucose in the absence and presence of 1-20 μg/ml PCA. The HG exposure of MC caused substantial increases in connective tissue growth factor (CTGF) expression and collagen IV secretion with mesangial hyperplasia, which were repealed by adding PCA. PCA boosted HG-plummeted membrane type-1 matrix metalloproteinase expression and dampened HG-elevated tissue inhibitor of matrix metalloproteinase-2 expression through disturbing transforming growth factor β (TGF-β)-SMAD signaling, facilitating extracellular matrix degradation. This study further revealed that PCA ameliorated HG-inflamed mesangial inflammation accompanying induction of intracellular cell adhesion molecule-1 and monocyte chemoattractant protein-1 (MCP-1) responsible for CTGF expression. The induction of intracellular cell adhesion molecule-1 and MCP-1 was mediated via TGF-β signaling, which was suppressed by PCA. In addition, the HG-promoted CTGF expression entailed nuclear factor κB (NF-κB) signaling involved in MCP-1 transcription. The HG-TGF-β induction was blocked in the presence of a NF-κB inhibitor, and the nuclear NF-κB translocation was blunted by a TGF-β receptor 1 inhibitor. PCA dampened NF-κB translocation in HG-exposed MC. These results demonstrate that there was a crosstalk between TGF-β-SMAD and NF-κB pathways in the diabetes-associated mesangial fibrosis and inflammation, which appeared to be severed by PCA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 23, Issue 4, April 2012, Pages 320-331
Journal: The Journal of Nutritional Biochemistry - Volume 23, Issue 4, April 2012, Pages 320-331
نویسندگان
Jing Li, Soon Sung Lim, Jae-Yong Lee, Jin-Kyu Kim, Sang-Wook Kang, Jung-Lye Kim, Young-Hee Kang,