کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8337989 | 1540973 | 2017 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular evaluation of vitamin D responsiveness of healthy young adults
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کلمات کلیدی
ELLCathelicidin antimicrobial peptideCLIAVDRVitamin D3 supplementationpTHPBMCsRAP2B1,25(OH)2D3 or 1,25DPBMCGAPDHqPCRTSS1α,25-dihydroxyvitamin D3 - 1α، 25-دی هیدروکسوییتامین D31,25(OH)2D3 - 1،25 (OH) 2D325(OH)D3 - 25 (OH) D325-hydroxyvitamin D3 - 25 هیدروکسی ویتامین D3cAMP - cAMPFAIRE - ایجادchemiluminescence immunoassay - ایمونویسی شیمیاییformaldehyde-assisted isolation of regulatory elements - جداسازی فرمالدئید از عناصر نظارتیtranscription start site - رونویسی شروع سایتPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیbody mass index - شاخص توده بدنBMI - شاخص توده بدنیMyoglobin - میوگلوبینparathyroid hormone - هورمون پاراتیروئیدquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیVitamin D3 - ویتامین D3، کوله کلسیفرولVitamin D receptor - گیرنده ویتامین D
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Vitamin D3 has via its metabolites 25-hydroxyvitamin D3 (25(OH)D3) and 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) direct effects on the transcriptome and the epigenome of most human cells. In the VitDbol study we exposed 35 healthy young adults to an oral vitamin D3 dose (2000 μg) or placebo and took blood samples directly before the supplementation as well as at days 1, 2 and 30. Within 24 h the vitamin D3 intake raised the average serum levels of both 25(OH)D3 and 1,25(OH)2D3 by approximately 20%. However, we observed large inter-individual differences in these serum levels, reflected by the average ratios between 25(OH)D3 and 1,25(OH)2D3 concentrations ranging from 277 to 1365. Interestingly, average serum parathyroid hormone (PTH) levels increased at day 1 by some 10% but then decreased within the following four weeks to levels 5% below baseline. In peripheral blood mononuclear cells (PBMCs) that were isolated at the same time points we determined vitamin D-modulated chromatin accessibility by FAIRE-qPCR at selected genomic loci. This method is well suited to evaluate both short-term and long-term in vivo effects of vitamin D on the epigenome of human subjects. The differential vitamin D responsiveness of the VitDbol study participants was determined via individual changes in their PTH levels or chromatin accessibility in relation to alterations in 25(OH)D3 concentrations. This led to the segregation of the subjects into 14 high, 11 mid and 10 low responders. In summary, the vitamin D responsiveness classification provides additional information compared to a vitamin D status assessment based on single 25(OH)D3 serum measurements. The study was registered at Clinicaltrials.gov (NCT02063334).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 174, November 2017, Pages 314-321
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 174, November 2017, Pages 314-321
نویسندگان
Sabine Seuter, Jyrki K. Virtanen, Tarja Nurmi, Jussi Pihlajamäki, Jaakko Mursu, Sari Voutilainen, Tomi-Pekka Tuomainen, Antonio Neme, Carsten Carlberg,