Effects of nuclear receptor transactivation on steroid hormone synthesis and gene expression in porcine Leydig cells

► Transactivation of CAR, PXR, or FXR in isolated Leydig cells using known agonists. ► Expression of several genes increased including CYB5A, CYB5R1, HSD17B4, and RDH12. ► Transactivation of CAR, PXR or FXR decreased sex steroid production. ► Transactivation of CAR or PXR increased 16-androstene steroid production.