کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8340747 1541252 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
oxBS-450K: A method for analysing hydroxymethylation using 450K BeadChips
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
oxBS-450K: A method for analysing hydroxymethylation using 450K BeadChips
چکیده انگلیسی
DNA methylation analysis has become an integral part of biomedical research. For high-throughput applications such as epigenome-wide association studies, the Infinium HumanMethylation450 (450K) BeadChip is currently the platform of choice. However, BeadChip processing relies on traditional bisulfite (BS) based protocols which cannot discriminate between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Here, we report the adaptation of the recently developed oxidative bisulfite (oxBS) chemistry to specifically detect both 5mC and 5hmC in a single workflow using 450K BeadChips, termed oxBS-450K. Supported by validation using mass spectrometry and pyrosequencing, we demonstrate reproducible (R2 > 0.99) detection of 5hmC in human brain tissue using the optimised oxBS-450K protocol described here.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 72, 15 January 2015, Pages 9-15
نویسندگان
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