کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8341092 | 1541279 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fractal analysis of extra-embryonic vessels of chick embryos under the effect of glucosamine and chondroitin sulfates
ترجمه فارسی عنوان
تجزیه فراکتال عروقهای غدد جنینی جنین های جوجه تحت تاثیر گلوکزامین و سولفات های کندرویتین
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Like heparan sulfate proteoglycans, some monosaccharides and glycosaminoglycans, such as sulfated glucosamine (GS) and chondroitin (CS), integrate the vascular extracellular matrix and may influence vascular endothelial cell growth. To assess the effects of these substances on blood vessel formation, we used the chick yolk sac membrane (YSM) model and fractal geometry quantification, which provided an objective in vivo method for testing potential agents that promote vasculogenesis and angiogenesis. An image processing method was developed to evaluate YSM capillary vessels after they were implanted in a methylcellulose disk of GS or CS at a concentration between 0.001-0.1Â mg/disk (performed on 2-day old embryos). This method resulted in a binary image of the microvascular network (white vessels on a black background). Fractal box-counting (DBC) and information (DINF) dimensions were used to quantify the activity of GS and CS in vasculogenesis and angiogenesis. YSM treated with GS (0.001-0.1Â mg) and CS (0.03-0.1Â mg) showed an increase in fractal dimensions that corresponded to vitelline vessel growth compared to the control group (vehicle), with GS displaying higher fractal dimension values.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 105, May 2016, Pages 114-118
Journal: Microvascular Research - Volume 105, May 2016, Pages 114-118
نویسندگان
Fernanda Katharine de Souza Lins Borba, Giovanni Loos Queiroz Felix, Edbhergue Ventura Lola Costa, Lisie Silva, Paulo Fernando Dias, Romildo de Albuquerque Nogueira,