کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8344208 | 1541564 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Experimental evidence of oxidative stress in plasma of homocystinuric patients: A possible role for homocysteine
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کلمات کلیدی
PBSGMDAMTHFRHcyCBSTAS - IASROS - ROSOxidative damage - آسیب اکسیداتیوOxidative stress - تنش اکسیداتیوAntioxidant defenses - دفاع از آنتیاکسیدانcystathionine β-synthase - سیستاتیونین β-سنتازmalondialdehyde - مالون دی آلدهیدMethionine - متیونینMET - ملاقات کردHomocystinuria - هموسیستینوریhomocysteine - هوموسیستئینTotal antioxidant status - وضعیت آنتی اکسیدان کلPlasma - پلاسماReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Homocystinuria is an inherited disorder biochemically characterized by high urinary excretion of homocystine and increased levels of homocysteine (Hcy) and methionine in biological fluids. Affected patients usually have a variety of clinical and pathologic manifestations. Previous experimental data have shown a relationship between Hcy and oxidative stress, although very little was reported on this process in patients with homocystinuria. Therefore, in the present study we evaluated parameters of oxidative stress, namely carbonyl formation, malondialdehyde (MDA) levels, sulfhydryl content and total antioxidant status (TAS) in patients with homocystinuria at diagnosis and under treatment with a protein restricted diet supplemented by pyridoxine, folate, betaine, and vitamin B12. We also correlated plasma Hcy and methionine concentrations with the oxidative stress parameters examined. We found a significant increase of MDA levels and carbonyl formation, as well as a reduction of sulfhydryl groups and TAS in plasma of homocystinuric patients at diagnosis relatively to healthy individuals (controls). We also verified that Hcy levels were negatively correlated with sulfhydryl content and positively with MDA levels. Furthermore, patients under treatment presented a significant reduction of the content of MDA, Hcy and methionine concentrations relatively to patients at diagnosis. Taken together, the present data indicate that lipid and protein oxidative damages are increased and the antioxidant defenses diminished in plasma of homocystinuric patients, probably due to increased reactive species elicited by Hcy. It is therefore presumed that oxidative stress participates at least in part in the pathogenesis of homocystinuria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Genetics and Metabolism - Volume 104, Issues 1â2, SeptemberâOctober 2011, Pages 112-117
Journal: Molecular Genetics and Metabolism - Volume 104, Issues 1â2, SeptemberâOctober 2011, Pages 112-117
نویسندگان
Camila Simioni Vanzin, Giovana Brondani Biancini, Angela Sitta, Carlos Alberto Yasin Wayhs, Izabela Netto Pereira, Francieli Rockenbach, Solange Cristina Garcia, Angela Terezinha de Souza Wyse, Ida Vanessa Doederlein Schwartz, Moacir Wajner,