کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8345912 | 1541624 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Redox conversions of dinitrosyl iron complexes with natural thiol-containing ligands
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Using the electron paramagnetic resonance (EPR) and optical spectrophotometric methods, it has been established that biologically active, water-soluble dinitrosyl iron complexes (DNIC) with glutathione are predominantly represented by the diamagnetic binuclear form (B-DNIC) even in the presence of a 10-fold excess of glutathione non-incorporated into DNIC at neutral pH. With the increase in ÑÐ to 10-11, B-DNIC are fully converted into the paramagnetic mononuclear form (Ð-DNIC) with a characteristic EPR signal at gâ¥Â = 2.04, gâ = 2.014 and gaver. = 2.03. After treatment with a strong reducing agent sodium dithionite, both Ð- and B-DNIC are converted into the paramagnetic form with a characteristic EPR signal at gâ¥Â = 2.01, gâ = 1.97 and gaver. = 2.0. Both forms display similar absorption spectra with absorption bands at 960 and 640 nm and a bend at 450 nm. After oxidation by atmospheric oxygen, this situation is reversed, which manifests itself in the disappearance of the EPR signal at gaver. = 2.0 and complete regeneration of initial absorption spectra of Ð- or B-DNIC with characteristic absorption bands at 390 or 360 and 310 nm, respectively. Treatment of bovine serum albumin (BSA) solutions with gaseous NO in the presence of Fe2+ and cysteine yields BSA-bound Ð-DNIC (Ð-DNIC-BSA). After treatment with sodium dithionite, the latter undergo transformations similar to those established for low-molecular Ð-DNIC with glutathione. Based on the complete coincidence of the optical and the EPR characteristics of sodium dithionite-treated Ð- and B-DNIC and other findings, it is suggested that sodium dithionite-reduced B-DNIC are subject to reversible decomposition into Ð-DNIC. The reduction and subsequent oxidation of Ð- and B-DNIC are interpreted in the paradigm of the current concepts of the initial electronic configurations of Ð- and B-DNIC (d7 ({Fe(NO)2}7) and d7-d7 ({Fe(NO)2}7-{Fe(NO)2}7), respectively).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 35, 30 November 2013, Pages 35-41
Journal: Nitric Oxide - Volume 35, 30 November 2013, Pages 35-41
نویسندگان
Rostislav R. Borodulin, Lyudmila N. Kubrina, Vladimir A. Serezhenkov, Dosymzhan S. Burbaev, Vasak D. Mikoyan, Anatoly F. Vanin,