کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8353204 | 1541894 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of WRKY transcription factors and characterization of two Botrytis cinerea-responsive LrWRKY genes from Lilium regale
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
plant defensin 1.2NLSsORFWRKYGFPPR proteinTFsqRT-PCR2-(N-morpholino) ethanesulfonic acid - 2- (N-مورفولینو) اتان سولفونیک اسیدEthylene - اتیلنSalicylic acid - اسید سالیسیلیکLOX - اکسیژن مایعreal-time quantitative PCR - زمان واقعی PCR کمیnuclear localization signals - سیگنال های محلی سازی هسته ایTranscription factor - عامل رونویسیTranscription factors - عوامل رونویسیopen reading frame - قاب خواندن بازlipoxygenase - لیپواکسیژنازMethyl jasmonate - متیل جاسموناتMeS - مسMeJA - میاgreen fluorescent protein - پروتئین فلورسنت سبزPathogenesis-related protein - پروتئین مرتبط با پاتوژنزBotrytis cinerea - کپک خاکستری
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A major constraint in producing lilies is gray mold caused by Botrytis elliptica and B. cinerea. WRKY transcription factors play important roles in plant immune responses. However, limited information is available about the WRKY gene family in lily plants. In this study, 23 LrWRKY genes with complete WRKY domains were identified from the Botrytis-resistant species Lilium regale. The putative WRKY genes were divided into seven subgroups (Group I, IIa-e, and III) according to their structural features. Sequence alignment revealed that LrWRKY proteins have a highly conserved WRKYGQK domain and a variant, the WRKYGKK domain, and these proteins generally contained similar motif compositions throughout the same subgroup. Functional annotation predicted they might be involved in biological processes related to abiotic and biotic stresses. A qRT-PCR analysis confirmed that expression of six LrWRKY genes in L. regale or the susceptible Asian hybrid 'Yale' was induced by B. cinerea infection. Among these genes, LrWRKY4, LrWRKY8 and LrWRKY10 were expressed at a higher level in L. regale than 'Yale', while the expression of LrWRKY6 and LrWRKY12 was lower in L. regale. Furthermore, LrWRKY4 and LrWRKY12 genes, which also respond to salicylic acid (SA) and methyl jasmonate (MeJA) treatments, were isolated from L. regale. Subcellular localization analysis determined that they were targeted to the nucleus. Constitutive expression of LrWRKY4 and LrWRKY12 in Arabidopsis resulted in plants that were more resistant to B. cinerea than wild-type plants. This resistance was coupled with the transcriptional changes of SA and JA-responsive genes. Overall, our study provides valuable information about the structural and functional characterization of LrWRKY genes that will not only deepen our understanding of the molecular mechanisms underlying the defense of lily against B. cinerea but also offer potential targets for cultivar improvement via biotechnology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 127, June 2018, Pages 525-536
Journal: Plant Physiology and Biochemistry - Volume 127, June 2018, Pages 525-536
نویسندگان
Qi Cui, Xiao Yan, Xue Gao, Dong-mei Zhang, Heng-bin He, Gui-xia Jia,