کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8354557 | 1541924 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanistic study of programmed cell death of root border cells of cucumber (Cucumber sativus L.) induced by copper
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کلمات کلیدی
PBSterminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelingFDA2′, 7′-dichlorodihydrofluorescein diacetateRBCsPCDMTPJC-1ΔΨmH2DCFDA - H2DCFD بهROS - ROSTUNEL - تونلfluorescein diacetate - دی فسفات فلورسینphosphate buffer saline - فسفات بافر شورProgrammed cell death - مرگ برنامهریزی شده یاختهprogrammed cell death (PCD) - مرگ سلولی برنامه ریزی شده (PCD)Copper - مسCopper (Cu) - مس (Cu)Mitochondrial membrane potential - پتانسیل غشای میتوکندریReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Programmed cell death (PCD) in root border cells (RBCs) induced by Copper (Cu) has been little studied. This study explored whether Cu induced PCD in RBCs of cucumber or not and investigated the possible mechanisms. The results showed that the percentage of apoptotic and necrotic RBCs increased with increasing concentration of Cu treatment. A quick burst of ROS in RBCs was detected, while mitochondrial membrane potential (ÎΨm) decreased sharply with Cu treatment. Caspase-3 like protease activity showed a tendency of increase with Cu treatment. The potential of Cu to induce PCD in RBCs of cucumber was first proved. Our results showed that ROS generation and mitochondrial membrane potential loss played important roles in Cu-induced caspase-3-like activation and PCD in RBCs of cucumber, which provided new insight into the signaling cascades that modulate Cu phytotoxicity mechanism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 97, December 2015, Pages 412-419
Journal: Plant Physiology and Biochemistry - Volume 97, December 2015, Pages 412-419
نویسندگان
Lijuan Sun, Jie Song, Cheng Peng, Chen Xu, Xiaofeng Yuan, Jiyan Shi,