کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8355205 | 1541948 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of ascorbic acid biosynthesis in different tissues of three non-heading Chinese cabbage cultivars
ترجمه فارسی عنوان
مقایسه بیوسنتز اسید آسکوربیک در بافت های مختلف سه رقم کلم بروکلی چینی
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کلمات کلیدی
ASAl-Galactono-1,4-lactonel-Gulono-1,4-lactoneAscorbic acid (vitamin C)PMMGDP-l-galactose phosphorylaseD-galacturonic acidl-galactose dehydrogenaseGGPmonodehydroascorbate reductaseGLDHGMEGMPqRT-PCRD-glucosedehydroascorbate reductaseDHARAAOAPXGDHMDHARGPPl-Galactono-1,4-lactone dehydrogenase - l-Galactono-1،4-lactone dehydrogenasel-Galactose - l-گالاکتوزascorbate oxidase - آسکوربات اکسیدازAscorbic acid - آسکوربیک اسیدd-Glucuronic acid - اسید d-گلوکورونیکPMI - شرکتهای کوچک و متوسطPhosphomannomutase - فسفامانوموتازMyo-inositol - میو-آنزیتولquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیascorbate peroxidase - پراکسیداز آسکورباتیNon-heading Chinese cabbage - کلم چینی غیر چینیglutathione reductase - گلوتاتیون ردوکتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Ascorbic acid (l-AsA) is an important antioxidant in plants and humans. Vegetables are one of the main sources of ascorbic acid for humans. For instance, non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is considered as one of the most important vegetables in south China. To elucidate the mechanism by which AsA accumulates, we systematically investigated the expression profiles of d-mannose/l-galactose pathway-related genes. We also investigated the recycling-related genes and AsA contents in different tissues of three non-heading Chinese cabbage cultivars, 'Suzhouqing', 'Wutacai' and 'Erqing' containing different amounts of AsA. Our results showed that six genes [d-mannose-6-phosphate isomerase 1 (PMI1), GDP-l-galactose phosphorylase 1 (GGP1), GGP2, GGP4, GDP-mannose-3â², 5â²-epimerase1 (GME1), and GME2] were expressed at high level and ascorbate oxidase (AAO) was expressed at low level. This expression pattern contributes, at least partially, to higher AsA accumulation in the leaves and petioles than in the roots. Eight genes (PMI1, GME, GGP, l-galactose-1-phosphate phosphatase, l-galactose dehydrogenase, l-galactono-1, 4-lactone dehydrogenase, monodehydroascorbate reductase 1, and glutathione reductase1) were also expressed at high level; AAO and ascorbate peroxidase (APX) were expressed at low level. This expression pattern may similarly contribute to higher AsA accumulation in 'Wutacai' and 'Suzhouqing' than in 'Erqing'. Therefore, the high expression levels of PMI, GME, and GGP and the low expression level of AAO contributed to the high AsA accumulation in non-heading Chinese cabbage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 73, December 2013, Pages 229-236
Journal: Plant Physiology and Biochemistry - Volume 73, December 2013, Pages 229-236
نویسندگان
Jun Ren, Zhongwen Chen, Weike Duan, Xiaoming Song, Tongkun Liu, Jianjun Wang, Xilin Hou, Ying Li,