کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356216 | 1542015 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Clade Ib basic helix-loop-helix transcription factor, bHLH101, acts as a regulatory component in photo-oxidative stress responses
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کلمات کلیدی
ASAPYERTSAPXBPDSFCRbHLHq-PCR - Q-PCRROS - ROSAscorbic acid - آسکوربیک اسیدferric chelate reductase - آهن کلات ردوکتازbasic helix-loop-helix - اسلحه پایه حلقه ایFER - انجام دهیدhigh light - برجستهbathophenanthroline disulfonate - حمام فنتانرولین دیسولفوناتMethylviologen - متیلویولوژنیFIT - مناسبquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیascorbate peroxidase - پراکسیداز آسکورباتیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The accumulation of reactive oxygen species (ROS) leads to oxidative damage; however, ROS also acts as signaling molecules. We previously demonstrated that the inducible silencing of thylakoid membrane-bound ascorbate peroxidase Arabidopsis plants (IS-tAPX) accumulated H2O2 in their chloroplasts, resulting in the clarification of ROS-responsive genes. In IS-tAPX plants, the transcript levels of the basic helix-loop-helix (bHLH) transcription factor bHLH101, which belongs to clade Ib bHLH, were down-regulated. In order to investigate the participation of bHLH101 in chloroplastic H2O2-mediated signaling, we isolated dominant negative expression mutants of bHLH101 (DN-bHLH101). DN-bHLH101 plants showed a significant phenotype that was sensitive to a methylviologen treatment, even under iron-sufficient conditions. Furthermore, the knock out mutant of bHLH101 showed a photo-oxidative sensitive phenotype, indicating that other clade Ib bHLHs do not compensate for the function of bHLH101. Thus, bHLH101 appears to act as a regulatory component in photo-oxidative stress responses. We also found that ferric chelate reductase activity was stronger in IS-tAPX plants than in control plants, suggesting that there is a close relationship between iron metabolism and oxidative stress responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 274, September 2018, Pages 101-108
Journal: Plant Science - Volume 274, September 2018, Pages 101-108
نویسندگان
Masahiro Noshi, Noriaki Tanabe, Yutaka Okamoto, Daisuke Mori, Masaru Ohme-Takagi, Masahiro Tamoi, Shigeru Shigeoka,