کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356570 | 1542019 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phytic acid transport in Phaseolus vulgaris: A new low phytic acid mutant in the PvMRP1 gene and study of the PvMRPs promoters in two different plant systems
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کلمات کلیدی
EMSInsPsPtdInsInsP6LPAPAPMRPABCinorganic phosphorousGUSPhaseolus vulgarisINSβ-glucuronidase - β-گلوکورونیدازEthyl methane sulfonate - اتیل متان سولفوناتLow phytic acid - اسید فیتیک پایینinositol phosphates - فسفات inositolphosphatidylinositol - فسفاتیدیل اینوزیتولPhosphorous - فسفرPhytic acid - فیتیک اسید، هگزوفسفات اینوزیتولHIP - مفصل ران یا مفصل هیپMyo-inositol - میو-آنزیتولmultidrug resistance protein - پروتئین مقاوم در برابر چندین رژیمATP-binding cassette - کیت اتصال به ATP
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phytic acid (InsP6) is the main storage form of phosphate in seeds. In the plant it plays an important role in response to environmental stress and hormonal changes. InsP6 is a strong chelator of cations, reducing the bioavailability of essential minerals in the diet. Only a common bean low phytic acid (lpa1) mutant, affected in the PvMRP1 gene, coding for a putative tonoplastic phytic acid transporter, was described so far. This mutant is devoid of negative pleiotropic effects normally characterising lpa mutants. With the aim of isolating new common bean lpa mutants, an ethyl methane sulfonate mutagenized population was screened, resulting in the identification of an additional lpa1 allele. Other putative lpa lines were also isolated. The PvMRP2 gene is probably able to complement the phenotype of mutants affected in the PvMRP1 gene in tissues other than the seed. Only the PvMRP1 gene is expressed at appreciable levels in cotyledons. Arabidopsis thaliana and Medicago truncatula transgenic plants harbouring 1.5â¯kb portions of the intergenic 5â² sequences of both PvMRP genes, fused upstream of the GUS reporter, were generated. GUS activity in different organs suggests a refined, species-specific mechanisms of regulation of gene expression for these two PvMRP genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 270, May 2018, Pages 1-12
Journal: Plant Science - Volume 270, May 2018, Pages 1-12
نویسندگان
Eleonora Cominelli, Massimo Confalonieri, Martina Carlessi, Gaia Cortinovis, Maria Gloria Daminati, Timothy G. Porch, Alessia Losa, Francesca Sparvoli,